How many times have you encountered a technical problem in your work that you needed to solve? Maybe it was an issue of workflow efficiency—too many samples, but too little time for hands-on work. Or maybe there wasn’t a technology available for what you needed to accomplish, and you didn’t have time to develop something […]
A widely used molecular biology technique, in vitro transcription uses bacteriophage DNA-dependent RNA polymerases to synthesize template-directed RNA molecules. Enzymes like bacteriophage SP6, T3 and T7 RNA polymerases are used to produce synthetic RNA transcripts, which can be used as hybridization probes, as templates for in vitro translation applications, or in structural studies (X-ray crystallography […]
One of the most critical parts of a Next Generation Sequencing (NGS) workflow is library preparation and nearly all NGS library preparation methods use some type of size-selective purification. This process involves removing unwanted fragment sizes that will interfere with downstream library preparation steps, sequencing or analysis. Different applications may involve removing undesired enzymes and […]
Aberrant methylation events have significant impacts in terms of incidence of cancer and development disregulation. Researchers studying DNA methylation are often working with DNA from “difficult” tissues such as formalin-fixed, paraffin embedded tissues, which characteristically yield DNA that is more fragmented than that purified from fresh tissue. Traditional methods for bisulfite conversion involve a long […]
Working with RNA can be a tricky thing…it falls apart easily, and RNases (enzymes that degrade RNA) are ubiquitous. Successfully isolating RNA and maintaining its integrity is critical, especially when sensitive downstream applications are used (e.g., RNA-Seq). Good techniques for RNA handling are simple to employ but crucial for success. All RNA purification and handling […]
“The Andromeda Strain”, a novel written by Michael Crichton, remains one of my favorite science fiction novels for two reasons (spoiler alert for the plot): The US government deliberately sent objects into space to scoop up extraterrestrial microorganisms and examine their potential to be used as a weapon (with the expected consequences of contaminated space […]
mRNA-based therapeutics are being explored across a range of applications, including vaccines, protein replacement and immunotherapies (2). Before any formulation decisions enter the picture, teams need confidence in the RNA itself: that it is the right sequence, right properties and the right purity to behave predictably downstream. That is where it helps to separate drug […]
Traditional protein study methods often disrupt the cellular context, impairing functional analysis. CRISPR/Cas9-mediated knock-in allows precise endogenous tagging, enhancing observation of proteins under native conditions. The HiBiT and NanoLuc® technologies enable sensitive, real-time analysis, crucial for drug discovery, offering efficient, scalable solutions for modern molecular biology challenges.
World Wildlife Conservation Day on December 4 emphasizes global efforts to protect endangered species and ecosystems. It highlights the significant threats of wildlife crimes and the role of science in biodiversity conservation. Practical actions for individuals include supporting sustainable products, reducing plastic use, and volunteering in local conservation initiatives.
For research use only Introduction: Diagnostic Innovation for Zoonotic Threats When a veterinarian detects influenza A in pigs, they’re not just protecting a herd; they’re helping safeguard public health through broad ongoing surveillance. To support rapid, biosafe detection of Influenza A viruses (H5N1, H3N2, H1N1) in animal populations, Promega and Longhorn Vaccines and Diagnostics have […]
