NanoLuc: Tiny Tag with a Big Impact

Synthetic biology—genetically engineering an organism to do or make something useful—is the central goal of the iGEM competition each year. After teams conquer the challenge of cloning their gene, the next hurdle is demonstrating that the engineered gene is expressing the desired protein (and possibly quantifying the level of expression), which they may do using a reporter gene.

Reporters can also play a more significant role in iGEM projects when teams design their organism with reporter genes to detect and signal the presence of specific molecules, like environmental toxins or biomarkers. Three of the iGEM teams Promega sponsored this year opted to incorporate some version of NanoLuc® Luciferase into their projects.

NanoLuc® luciferase is a small monomeric enzyme (19.1kDa, 171 amino acids) based on the luciferase from the deep sea shrimp Oplophorus gracilirostris. This engineered enzyme uses a novel substrate, furimazine, to produce high-intensity, glow-type luminescence in an ATP-independent reaction. Unlike other molecules for tagging and detecting proteins, NanoLuc® luciferase is less likely to interfere with enzyme activity and affect protein production due to its small size.

NanoLuc® Luciferase has also been engineered into a structural complementation reporter system, NanoBiT® Luciferase, that contains a Large subunit (LgBiT) and two small subunit options: low affinity SmBiT and high affinity HiBiT. Together, these NanoLuc® technologies provide a bioluminescent toolbox that was used by the iGEM teams to address a diverse set of biological challenges.

Here is an overview of each team’s project and how they incorporated NanoLuc® technology.

Continue reading “NanoLuc: Tiny Tag with a Big Impact”

The Future of Synthetic Biology: A Recap of iGEM 2019

After attending the iGEM Giant Jamboree last year and being completely blown away by the projects presented (check out this article or this one), I didn’t think I’d be as astonished this year. I attributed part of the awe I felt over the caliber and quality of the projects to my wide-eyed naiveté, having never attended the event before. The second time around, the “first-time” novelty long worn off, I didn’t expect to feel that same level of amazement.

I couldn’t have been more wrong.

After three days of impressive presentations, I once again felt that same astonishment as I prepared to watch the presentations of the 6 finalists. With good reason—the projects presented by the six finalists completely blew my mind!

Continue reading “The Future of Synthetic Biology: A Recap of iGEM 2019”

Tales from the Trenches: Career Growth in Biotechnology

Building a successful career in the biotechnology industry is really just a series of transitions from one role to another. But the devil is in the details—when to make a change, how to create opportunities and who can be your champion as you pivot. So how do you navigate these factors to keep your career goals on course?

Bob Weiland answers a question posed by Michele Smith at the MS Biotech Alumni Symposium.

I recently attended a symposium (presented by the University of Wisconsin Master of Science in Biotechnology Program, of which I’m an alum) that addressed this topic through the lens of one individual with a storied career in the industry. Bob Weiland currently serves on the Board of Directors for CymaBay Therapeutics. He has held various roles, from sales and marketing to operations and strategy, within large, established companies (Abbot, Baxter, Takeda) and smaller ones (Pacira Pharmacueticals). He drew on this wide-ranging experience to provide advice to professionals at all career stages.

Bob began the talk by declaring that there will be points in your career when you reach a “hard spot” and will need to transition, whether to a new role, company or even industry, to meet your career goals. He suggested a good starting point is simply to be thinking about making a change. But in the same breath he emphasized, “What are you doing about it?” He identified four distinct actions that you can take to ensure role changes and career transitions support your professional growth and development.

Continue reading “Tales from the Trenches: Career Growth in Biotechnology”

In the Face of Failure, Create Your Own Path

Do you ever wonder whether you’re on the “right” career path? If you’re in academia, the trajectory you should follow can seem pretty rigid—undergrad degree, PhD, postdoc, PI, and then the elusive tenure. Have you considered that there isn’t a single “correct” path?

The hosts of HelloPhD, Josh Hall and Dan Arneman, interview Promega Science Writer Julia Nepper for their podcast.

That’s the message one of Promega’s Science Writers, Julia Nepper PhD, emphasized when she was interviewed recently on the HelloPhD podcast. The HelloPhD podcast offers advice to help students, postdocs, faculty and scientists navigate the hard questions they face every day related to graduate school and careers in science.

In Episode 121: A Teenager Goes to Grad School, Julia offered her insight on dealing with failure and finding a scientific career path that’s right for you. She also shared her unusual story of starting grad school at age 17 and some of the unique experiences she had along the way that led her to choose a career in scientific communications.

To listen to this podcast and learn more about HelloPhD, click here.

Related Posts

Sci Comm Tips From An iGEM Judge

Formal judgement in any context is nerve-racking. Scientists, familiar with being judged, rely on others to evaluate (and hopefully accept) everything from a PhD thesis defense to grant proposals and peer-reviewed journal article submissions. The frustrating part is not knowing exactly what the judges are looking for. Sure there are requirements and guidelines to follow—but how are the judges going to interpret them? It would be a whole lot easier if we could just peek into their minds. Unfortunately for most, that fantasy isn’t likely to turn into reality.

But if you are part of an iGEM team, today is your lucky day! Our own Preeta Guptan volunteers as a judge for the iGEM competition, and in today’s article you will get her insider’s perspective about what iGEM judges look for. You will also get some tips to help you excel in the iGEM competition—and effectively communicate about science in general.

Preeta is an External Innovation Manager at Promega, which means she seeks out and investigates technology that might be valuable for Promega to license or acquire. The opportunity to scout up-and-coming synthetic biology advances was one reason she wanted to be an iGEM judge, but curiosity was at the core of her decision. Preeta and the other judges bring their unique perspectives and experiences to each iGEM project and team they evaluate. Here are some suggestions from Preeta:

Continue reading “Sci Comm Tips From An iGEM Judge”

Immunotherapy—Don’t Forget the Microbiome

Bacteria make you sick. The idea that bacteria cause illness has become ingrained in modern society, made evident by every sign requiring employees to wash their hands before leaving a restroom and the frequent food recalls resulting from pathogens like E. coli. But a parallel idea has also taken hold. As microbiome research continues to reveal the important role that bacteria play in human health, we’re starting to see the ways that the microbiota of the human body may be as important as our genes or environment.

The story of how our microbiome affects our health continues to get more complex. For example, researchers are now beginning to understand that the composition of bacteria residing in your body can significantly impact the effects of therapeutic drugs. This is a new factor for optimizing drug response, compared to other considerations such as diet, interaction with other drugs, administration time and comorbidity, which have been understood much longer.

Continue reading “Immunotherapy—Don’t Forget the Microbiome”

Lab Sustainability: Easy as 1-2-3

Sustainability is a bit of buzzword lately—for good reason—but knowing how to be more sustainable and actually putting sustainable practices in action are not the same thing. This may be one reason why scientists have been slow to adopt change in their laboratories. By sponsoring My Green Lab, we’re hoping to help spread the message that there are simple changes researchers can make in their labs to significantly impact sustainability.

Here are some easy ways to reduce energy, water and waste in your lab and start making your research more sustainable.

1. Energy

Compared to office buildings on campus, academic lab buildings consume 5 times more energy. To put that into perspective, labs typically consume 50% of the energy on a university campus despite occupying less than 30% of the space. Fortunately, reducing energy usage can be one of the easiest ways to make your lab more sustainable. Continue reading “Lab Sustainability: Easy as 1-2-3”

Making Research More Sustainable, One Lab at a Time

Do you love your research job? What if you couldn’t do that work anymore? What if future researchers couldn’t have the opportunity to build from what you have accomplished and feel the same joy you do about their research?

Unfortunately, these may become more than hypotheticals for the next generation of scientists due to the impact humans are having on the earth. Scientific research has an outsized impact on some aspects of our unsustainable use of resources. Academic research buildings can use four times more energy than a typical office building and can be responsible for one-third of all waste generated on campus. So, can you make scientific research more sustainable? Continue reading “Making Research More Sustainable, One Lab at a Time”

Synthetic Biology by the Letters

Synthetic biology has been in the news a lot lately—or maybe it only seems like it because I’m spending a lot of my time thinking about our partnership with the iGEM Foundation, which is dedicated to the advancement of synthetic biology. As the 2019 iGEM teams are forming, figuring out what their projects will be and how to fund them, it seemed fitting to share some of these stories.

A, C, T, G…S, P, Z, B?

Researchers recently developed four synthetic nucleotides that, when combined with the four natural nucleotides (A, C, T and G), make up a new eight-letter synthetic system called “hachimoji” DNA. The synthetic nucleotides—S, P, Z and B— function like natural DNA by pairing predictably and evolving. Continue reading “Synthetic Biology by the Letters”

It’s Almost iGEM Season—Help Is On The Way!

The 2019 iGEM Competition is on the horizon and team registration opens this month. We’re excited to partner with the iGEM Foundation again this year and offer our support to the young scientists who participate. If you’re starting an iGEM project, there are going to be things you need along the way. We are pleased to share a number of different ways we can help your iGEM team from now until the Giant Jamboree.

Grant Sponsorship

Tell us about your iGEM project and your team could win a 2019 Promega iGEM Grant Sponsorship. Ten winning teams will each receive $2000 in free Promega products to use for their iGEM projects. Tell us about your project—What problem are you addressing? What is your proposed solution? What challenges does your team face? Last year’s winning teams selected from a wide range of reagents and supplies, including master mix, restriction enzymes, ligase, DNA purification kits, expression systems, DNA ladders and markers, buffers and agarose. Click here to apply! Continue reading “It’s Almost iGEM Season—Help Is On The Way!”