Understanding the expression, function and dynamics of
proteins in their native environment is a fundamental goal that’s common to
diverse aspects of molecular and cell biology. To study a protein, it must
first be labeled—either directly or indirectly—with a “tag” that allows
specific and sensitive detection.
Using a labeled antibody to the protein of interest is a
common method to study native proteins. However, antibody-based assays, such as
ELISAs and Western blots, are not suitable for use in live cells. These
techniques are also limited by throughput and sensitivity. Further, suitable
antibodies may not be available for the target protein of interest.
On April 5, 2025, Dr. David R. Liu stood in the spotlight at the Barker Hangar in Santa Monica, California, to receive the Breakthrough Prize in Life Sciences—one of the most prestigious honors in science. Dubbed the “Oscars of Science,” the Breakthrough Prizes were launched in 2012 by tech philanthropists including Sergey Brin, Mark Zuckerberg and Priscilla Chan, Yuri and Julia Milner and Anne Wojcicki. These prizes recognize groundbreaking achievements in life sciences, physics, and mathematics, with each laureate receiving a $3 million award—more than twice the amount of a Nobel Prize.
The winners are selected by panels of previous Breakthrough Prize recipients, ensuring peer-driven recognition. The annual ceremony brings together not only the best minds in science but also celebrities, filmmakers, and tech industry leaders, creating an uncommon crossover between pop culture and research, in an effort to bring more public attention as well as funding to scientific achievement.
Dr. Liu was honored for inventing base editing and prime editing, technologies that allow precise, programmable rewriting of DNA to correct mutations linked to genetic disease—without introducing double-stranded breaks. These tools have rapidly transitioned from the bench to the clinic, with at least 15 clinical trials currently underway worldwide targeting diseases like sickle cell anemia, T-cell leukemia, and others.
Almost three-quarters of the major crop plants across the globe depend on some kind of pollinator activity, and over one-third of the worldwide crop production is affected by bees, birds, bats, and other pollinators such as beetles, moths and butterflies (1). The economic impact of pollinators is tremendous: Between $235–577 billion dollars of global annual food production relies on the activity of pollinators (2). Nearly 200,000 species of animals act as pollinators, including some 20,000 species of bees (1). Some of the relationships between pollinators and their target plants are highly specific, like that between fig plants and the wasps that pollinate them. Female fig wasps pollinate the flowers of fig plants while laying their eggs in the flower. The hatched wasp larvae feed on some, but not all, of the seeds produced by fertilization. Most of the 700 fig plants known are each pollinated by only one or a few specific wasp species (3). These complex relationships are one reason pollinator diversity is critical.
Measuring the Success of Conservation Legislation
A bee pollinates the lavender flowers.
We are now beginning to recognize how critical pollinator diversity is to our own survival, and many governments, from the local level to the national level are enacting policies and legislation to help protect endangered or threatened pollinator species. However, ecosystems and biodiversity are complex subjects that make measuring and attributing meaningful progress on conservation difficult. Not only are there multiple variables in every instance, but determining the baseline starting point before the legislation is difficult. However, there are dramatic examples of success in saving species through legislative and regulatory action. The recovery of the bald eagle and other raptor populations in the United States after banning the use of DDT is one such example (4).
This review summarizes Valto Biocontrol’s move toward a high-throughput (HT) nucleic acid extraction solution utilizing Promega Maxwell® HT simplyRNA Kit, Custom. This project was initiated by Dr. Menno Westerdijk, Head of Laboratories at Valto Biocontrol, who established a molecular laboratory onsite. The Promega Field Support Scientist team assisted Dr. Westerdijk to develop an automated nucleic acid solution using their existing KingFisher™ Flex robot. Dr. Westerdijk’s extensive experience working with KingFisher robots in the agricultural sector combined with expert guidance from Promega Field Support enabled a seamless implementation of the Maxwell® HT chemistry for his laboratory.
In January 2024, Antonio Alcamí and Ángela Vázquez, virologists from the Severo Ochoa Centre for Molecular Biology, landed in Antarctica to study the avian flu virus. They embarked on a journey to monitor 17,000 penguins as part of their efforts to study the virus and prevent its spread. Our Maxwell® RSC 48 was delivered to extract nucleic acids from the samples, which are set to be analyzed using qPCR.
In the Spring of 2015, greenhouse tomato plants grown in Jordan presented with a mosaic pattern of light and dark green patches on leaves, narrowing leaves, and yellow- and brown-spotted fruit (Salem et al. 2015). The pathogen was identified as a novel plant virus, the tomato brown rugose fruit virus (ToBRFV), and the original outbreak was traced back to the fall of 2014 to Israel (Luria et al. 2017). This newly emerging virus can infect tomato and pepper plants at any stage of development and greatly affect crop yield and quality. Furthermore, the virus spreads rapidly by mechanical contact but can also be spread over long distances by contaminated seeds (Caruso et al. 2022), and as of 2022 it had been detected in 35 countries across four continents (Zhang et al. 2022). Compounding its transmissibility, is the ability of the virus escape plant genetic resistance to viral infection (Zhang et al. 2022). There are seven host plants for the virus, including some common grasses and weeds, which could act as a reservoir for the virus, even if it is eliminated from commercial crops. Some researchers consider ToBRFV to be the most serious threat to tomato production in the world.
Farmers everywhere strive to protect their crops and ensure a stable food supply while minimizing environmental harm. A promising approach to achieving this leverages a plant’s built-in defense mechanisms, reducing the need for chemical interventions. Many geneticists and agronomists lean on technologies that can automate and streamline nucleic acid extraction and pathogen detection to identify naturally pest resistant crops and, ultimately, keep up with the changing agricultural landscape.
One key obstacle to crafting effective gene therapies is the ability to tailor dosing according to a patient’s needs. This can be tricky because even if protein production is successful, staying within the therapeutic window is paramount—too much of a protein could be toxic, and too little will not produce the desired effect. This balance is difficult to achieve with current technologies. In a study recently published in Nature Biotechnology, researchers at Baylor College of Medicine investigated a possible solution to this problem, engineering a molecular “on/off” switch that could regulate gene expression and maintain protein production at dose-dependent, therapeutic levels.
Sally Seraphin and her students Maliah Ryan (second from right) and Jude Altman (right) work with a Promega Applications Scientist at the Marine Biological Laboratory
Sally Seraphin’s life in the research lab started with rats and roseate terns. Chimpanzees and rhesus macaques came next, then humans (and a brief foray into voles). When she pivoted to red-eyed tree frogs, Sally once again had to learn all kinds of new techniques. Suddenly, in addition to new sample prep and analysis techniques, she needed to get up to speed on amphibian care and husbandry. That led her to the Marine Biological Laboratory (MBL) in Woods Hole, MA.
“It’s a seaside resort atmosphere with experts in every technology you can imagine,” Sally says. “It’s a place to incubate and birth new approaches to answering questions.”
Sally spent the past two summers at MBL learning everything she needed to know about breeding and caring for amphibians. During that time, she also worked closely with Applications Scientists from Promega who helped her start extracting RNA from frog samples.
“The hands-on support from industry scientists is definitely unique to Promega and MBL,” she says. “It’s rare to have a specialist on hand who can help you learn, troubleshoot and optimize in such a finite amount of time.”
Adopting a New Model Organism
Sally uses red-eyed tree frogs to study early stress and developmental timing. Photo from Wikimedia.
Sally studies how early stress impacts brain and behavior development. She hopes to deepen our understanding of how adverse childhood experiences connect to mental illness and bodily disease later in life. In the past, she studied how factors such as parental absence affected the neurotransmission of dopamine in primates. Recently, she changed her focus to developmental timing.
“Girls who are exposed to early trauma like sexual or physical abuse will sometimes reach puberty earlier than girls who aren’t,” Sally explains. “And I noticed that there are many species that will alter their developmental timing in response to predators or social and ecological threats.”
In the evolving field of forensic science, a study by Fantinato et al. has opened new avenues in using DNA extraction and analysis to recover important information from crime scenes. Their work, “The Invisible Witness: Air and Dust as DNA Evidence of Human Occupancy in Indoor Premises,” focuses on extracting DNA from air and dust. This novel approach could revolutionize how crime scenes are investigated, especially in scenarios where traditional evidence—like fingerprints or bodily fluids—is scarce, degraded or has been removed from surfaces.
XWe use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To learn more about our approach to Privacy we invite you to Read More
By clicking “Accept All”, you consent to the use of ALL the cookies. However you may visit Cookie Settings to provide a controlled consent.
We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.
If you are located in the EEA, the United Kingdom, or Switzerland, you can change your settings at any time by clicking Manage Cookie Consent in the footer of our website.
Necessary cookies are absolutely essential for the website to function properly. These cookies ensure basic functionalities and security features of the website, anonymously.
Cookie
Duration
Description
cookielawinfo-checbox-analytics
11 months
This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics".
cookielawinfo-checbox-functional
11 months
The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional".
cookielawinfo-checbox-others
11 months
This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
cookielawinfo-checkbox-advertisement
1 year
The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Advertisement".
cookielawinfo-checkbox-necessary
11 months
This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary".
cookielawinfo-checkbox-performance
11 months
This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance".
gdpr_status
6 months 2 days
This cookie is set by the provider Media.net. This cookie is used to check the status whether the user has accepted the cookie consent box. It also helps in not showing the cookie consent box upon re-entry to the website.
lang
This cookie is used to store the language preferences of a user to serve up content in that stored language the next time user visit the website.
viewed_cookie_policy
11 months
The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data.
Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc.
Cookie
Duration
Description
SC_ANALYTICS_GLOBAL_COOKIE
10 years
This cookie is associated with Sitecore content and personalization. This cookie is used to identify the repeat visit from a single user. Sitecore will send a persistent session cookie to the web client.
vuid
2 years
This domain of this cookie is owned by Vimeo. This cookie is used by vimeo to collect tracking information. It sets a unique ID to embed videos to the website.
WMF-Last-Access
1 month 18 hours 24 minutes
This cookie is used to calculate unique devices accessing the website.
_ga
2 years
This cookie is installed by Google Analytics. The cookie is used to calculate visitor, session, campaign data and keep track of site usage for the site's analytics report. The cookies store information anonymously and assign a randomly generated number to identify unique visitors.
_gid
1 day
This cookie is installed by Google Analytics. The cookie is used to store information of how visitors use a website and helps in creating an analytics report of how the website is doing. The data collected including the number visitors, the source where they have come from, and the pages visted in an anonymous form.
Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. These cookies track visitors across websites and collect information to provide customized ads.
Cookie
Duration
Description
IDE
1 year 24 days
Used by Google DoubleClick and stores information about how the user uses the website and any other advertisement before visiting the website. This is used to present users with ads that are relevant to them according to the user profile.
test_cookie
15 minutes
This cookie is set by doubleclick.net. The purpose of the cookie is to determine if the user's browser supports cookies.
VISITOR_INFO1_LIVE
5 months 27 days
This cookie is set by Youtube. Used to track the information of the embedded YouTube videos on a website.
Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors.
Cookie
Duration
Description
YSC
session
This cookies is set by Youtube and is used to track the views of embedded videos.
_gat_UA-62336821-1
1 minute
This is a pattern type cookie set by Google Analytics, where the pattern element on the name contains the unique identity number of the account or website it relates to. It appears to be a variation of the _gat cookie which is used to limit the amount of data recorded by Google on high traffic volume websites.