Tardigrade Proteins Might Solve the Cold Chain Problem for Biologics

image depicting a microscopic tardigrade

Some of our most advanced medicines today rely on components derived from living organisms. These therapeutics, called biologics, include things like vaccines, blood products like Human Blood Clotting Factor VIII (FVIII), antibodies and stem cells. Biologics are incredibly temperature sensitive, which means they need to be kept cold during production, transport and storage, a process collectively called the cold chain. The stringent transport and storage temperature requirements for biologics create a barrier to accessing these lifesaving options; particularly for those in remote or underdeveloped regions, where maintaining a cold chain is logistically difficult and costly.

But what if we could break the cold chain? Inspired by one of the most resilient creatures on Earth – the tardigrade – scientists at the University of Wyoming are exploring ways to do just that.

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Promega Summer Interns Contribute Skills and Ideas from Logistics to R&D

Internships at Promega aren’t about getting coffee for your boss or shredding thousands of old papers. Promega interns take responsibility for complex projects that create notable impacts for their teams, our customers, or Promega as a whole.

Promega hosted 56 interns over the summer in 2024. These students came with unique skills in science, engineering, marketing, IT and so much more. We asked several of them to write about the work they did, as well as the results and benefits they created.

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High School Interns Get Hands-On Experience in Promega R&D

High school interns Tessara Clark and Evelyn Zadzilka work in the lab during their Maydm internships in Promega R&D
Tess (left) and Evie (right) spent the summer after high school graduation interning in Promega R&D.

If she weren’t working at Promega, Evie Zadzilka probably would’ve spent the summer after high school graduation taking summer classes before reporting to her freshman year at the University of Wisconsin-Madison. She runs a small art business, and she might’ve spent more time taking commissions.

Instead, Evie spent the summer before college as an intern in Promega R&D, honing her pipetting skills as she learns about primer design and contributing to the development of a new Promega assay.

“I’ve had a great time,” she says. “I’ll definitely take a lot with me from this experience. I’m so glad I got to do it.”

Evie and her fellow intern Tess Clark were the two high school-aged interns placed at Promega through a relationship with a Madison-based nonprofit called Maydm. This organization helps girls and youth of color in grades 6-12 prepare for careers in STEM by providing educational opportunities and experiences. Through school and summer programs, they strive to disrupt systemic barriers by empowering students like Evie to pursue their dreams as entrepreneurs, developers, engineers and more.

“This will really boost my confidence when I get into lab work next year,” Evie says.

High School Internships at Promega

Tessara Clark and Evelyn Zadzilka work in the lab during their Maydm high school internships in Promega R&D
Tess and Evie were placed in Promega internships through the non-profit organization Maydm.

During their senior year of high school, Tess and Evie were both enrolled in dual-credit courses through Madison College. These classes made them eligible to apply for a high school internship through Maydm.

“I’ve been interested in research for a very long time,” says Tess, another recent school graduate preparing to enter the University of Wisconsin-Madison. “I’m going to major in physics next year, and I don’t have many ties to the biotechnology or chemistry I’ve worked with at Promega. But I wanted hands-on lab experience, so that’s how I ended up here.”

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What Do You Get from a DOORS Scholarship?

D.O.O.R.S. Scholars Day 2024
Eight D.O.O.R.S. Scholars visiting Kornberg Center at Promega Madison in April 2024

The BioPharmaceutical Technology Center Institute (BTC Institute) is collaborating with Promega to provide D.O.O.R.S. Scholarships to 10 students from underrepresented backgrounds. The scholarship has been awarded annually since 2020.

Each student will receive a $5,000 scholarship. But what else can you expect from a D.O.O.R.S. Scholarship?

In April 2024, eight D.O.O.R.S. Scholars visited Promega Madison for D.O.O.R.S. Scholars Day. At the end of the day, they shared their thoughts on the benefits they gained from the program.

Mentorship

D.O.O.R.S. Scholars Lucas Lisondo and Julia Forsey working in the lab on D.O.O.R.S. Scholars Day 2024
Julia Forsey and Lucas Lisondo work in the lab during their visit to Promega Madison

Each D.O.O.R.S. Scholar is assigned a Promega scientist as a mentor. Throughout the school year, they participate in individual and group mentorship sessions that include constructive feedback on their research and professional development.      

My mentor, Sid Withers, was super influential to me. He walked me through differences in what it means to get a PhD or a Masters. We talked about different stigma and mental health issues surrounding science and academia. And overall, I really appreciated his insight into careers in the biotech industry.

Julia Forsey, McGill University
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A Peek Behind the Scenes: Young Researchers Visit Promega Madison

Young Researcher Award recipients 2024
Young Researcher Award winners Alexandre Lalande, Margaux Cochard and Emmanuel Heilman (L to R) visited Promega Madison in June 2024.

Earlier this summer, Promega Madison welcomed three rising researchers from Europe for an immersive experience on the company’s main campus. Alexandre Lalande (PhD student, International Center for Infectiology Research, France), Margaux Cochard (Post-Doc, University of the Littoral Opal Coast, France) and Emmanuel Heilman (Post-Doc, Medical University of Innsbruck) were selected as Young Researcher Award winners by Promega France and Promega Germany. Their prize trip to the United States included tours of Promega facilities, conversations with Promega leaders and research scientists and hands-on training with emerging technologies.

“It’s really interesting to see how Promega manages to find harmony between the science, the people and the business,” Alexandre says. “When you arrive here you immediately feel comfortable.”

Meeting Industry Scientists

Alexandre says that he has always imagined himself working in academia doing basic research, but he never totally ruled out opportunities in industry.

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The Marvel of Malate: A Crucial Component in Cellular Energy Metabolism

Today’s blog written by guest author Kim Haupt.

Cellular energy metabolism is a complex biological process that relies on a suite of metabolites, each with distinct roles to maintain. Malate is one of these metabolites and is essential for maintaining cellular function through important roles in both energy production and redox homeostasis. In this blog, we highlight malate’s diverse roles and uncover some of its connections to human disease. 

Illustration of energy metablism in cell.
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Elephants May Call Each Other By Name

Elephants intertwining trunks
New research points to the possible use of names among elephants in the African savanna

Names are part of something called “arbitrary communication”, wherein a sound represents an idea without imitating it. Where we have so far seen names used in nature, they are typically imitative—for instance, bottlenose dolphins and parrots mimic an individual’s signature call to get their attention. However, this differs from how humans refer to each other using an arbitrary or random sound chosen to represent an individual. The use of arbitrary language is a mark of linguistic complexity that moves beyond the limitations of imitative speech and expands expressive power into the potential exchange of intricate ideas, abstract concepts and symbolic thought.

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The Battle of Shiloh’s Angel’s Glow: Fact, Civil War Legend or Modern Myth? 

It sounds like the script for a Hollywood movie. The story, first appearing in 2001, begins with a purported civil war legend from the Battle of Shiloh. The legend said that the wounds of some soldiers glowed (faintly) in the dark. Soldiers with these glowing wounds were more apt to survive, giving the phenomenon the name “Angels Glow”. The story ends with two curious teenagers solving the mystery using their science fair project. They identify infection by the bioluminescent bacteria Photorhabdus luminescens (formerly Xenorhabdus luminescens) as the likely cause of the glowing wounds. P. luminescens produces bacteriocins (antimicrobial peptides), which the teenagers attribute to helping keep other infections at bay, resulting in the improved survival rate for the soldiers whose wounds glowed.

The teenagers win. The mystery is solved. The credits roll. 

Except life (and science) is rarely as simple as a summer block buster. 

Cannon at sunset on a civil war battlefield
The Battle of Shiloh took place in Hardin County Tennessee on April 6th and 7th, 1862.
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Revolutionizing Food Security: How Biotechnology Contributes to Sustainability and Safety

field of crops/food

Projections from the United Nations suggest that the global population reached 8 billion in 2022. By 2030, the United Nations expect the population will grow to 8.5 billion (1).  In order to sustain the rapidly expanding global population, innovative approaches in the agriculture sector are required to ensure food security and safety while maintaining sustainable practices.

Centuries of cultivating crops and raising livestock have honed our current agricultural methods. In the 21st century, these techniques encounter persistent challenges. Environmental factors such as soil degradation, water scarcity, and climate change pose significant threats to production. Additionally, the constant risks posed by pests and diseases can devastate both crops and livestock.

Read more about how the current avian flu crosses species and affects livestock.

The agriculture sector’s challenge of feeding the world sustainably lies in the limited access to natural resources like land and water. Unfortunately, these resources don’t grow with our population, so we need to find a way to increase productivity per unit of land (2). Ideally, using less water and potentially harmful pesticides.

Biotechnology offers innovative solutions that support sustainable agriculture practices to not only enhance food production, but also increase nutritional value and safety of our food supply.

Biotechnology in Agriculture: Enhancing Crop Yield and Resilience:

For much of the history of agriculture, breeding programs have involved selectively breeding desirable traits to increase yield, quality, and resilience. In the age of biotechnology, agriculturalists are revolutionizing this practice with the help of cloning and CRISPR technologies.

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Logistics Built to Deliver: Kepler Center and Beyond

In March 2024, Promega celebrated a significant milestone by completing extensive renovations to Kepler Center, the primary distribution warehouse located at Promega Madison. This massive expansion has increased the facility’s total area to an astounding 320,000 square feet (29,822 square meters).

So, what does this mean for you?

When you place an order from Promega, you can be confident your products will arrive on time. With customers in more than 120 countries, we have built a global logistics network that ensures quality and reliability from the warehouse to your lab. This expansion of Kepler Center enhances our ability to ensure prompt shipping, reaffirming our commitment to timely deliveries.

Delivering Products When You Need Them

Promega Madison ships directly to 40 countries. We maintain close relationships with domestic carriers and international freight forwarders to make sure packages are transported safely and efficiently. Some of these shipments go directly to customer labs, while others will stock distribution facilities around the world.

Promega has additional logistics warehouses strategically located around the world. These warehouses have much of the same capabilities as Kepler Center, such as a range of storage temperature capabilities including ambient, +4°C, -20°C, -70°C and liquid nitrogen cryogenic storage.

Our logistics teams around the world maintain local inventory and oversee the final delivery of orders. We share common processes around the world to ensure quality and continuity throughout the supply chain. These teams also work with our network of distributors to supply products to specific regions.

Our largest logistics facility outside the United States is the EuroHub, located in Walldorf, Germany. This 3,200 square foot (300 square meter) facility acts as a fulfillment agent, managing the entire logistics process to supply customers of every European branch. In 2023, almost 83,000 parcels were dispatched through the EuroHub.

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