Luciferase reporter assays are highly versatile, but their true power comes when the reporter system you’ve selected is well aligned with your experimental objectives. Whether you’re tracking transcriptional changes or pathway activity, assessing miRNA/siRNA regulation, or using as a readout in CRISPR-based screens, choosing a reporter and detection assay format that fit your specific research goal is critical for meaningful, reproducible results.

You may have already read about how to choose a luciferase reporter assay, but now, we will walk through how to match luciferase reporter systems—reporter types, detection chemistries, and formats—to your specific experimental needs. While luciferases like NanoLuc have applications beyond gene expression, this blog focuses on genetic reporter applications and the workflows that support them.

G-protein-coupled receptors (GPCRs) are among the most important drug targets in human biology, mediating signals across nearly every physiological system. But not all GPCRs are equally easy to study—especially those that interact with peptide ligands. These ligands tend to be flexible, fast-moving, and hard to trace in live cells by standard methods. Historically, radioligand binding assays have filled this gap, offering a way to measure peptide–receptor interactions with high sensitivity. However, these assays are typically performed using isolated membrane preparations or cells under non-physiological conditions, and they don’t allow for real-time or kinetic measurements.