Fixed in the Past, Focus on the Future

“I would do more with my samples, but it’s just not possible…I know there’s probably a wealth of information in there, but there is just no way to get it out…I’ve got blocks of tissue sitting in the lab, experiments I want to run, but no good way to get clean nucleic acids out.” These […]

Paying it Forward: A Promega Employee’s Experience With the American Chemical Society

If you are a scientist you know the American Chemical Society (ACS) for their high quality journals (all 39 of them) and for their annual meetings and conferences. But did you know the ACS also focuses on community education and outreach? The ACS mission is “Improving people’s lives through the transforming power of chemistry.” According […]

Cell-Free Applications:Protein Arrays (Nucleic Acid Programmable)

The traditional methods of generating protein arrays require the separate expression of hundreds of proteins, followed by purification and immobilization of the proteins on a solid surface. Cell-Free protein array technology produces protein microarrays by performing in vitro synthesis of the target protein from their DNA templates. One approach for the generation of cell- free […]

Cell Free Expression Application: Production of Soluble Protein for Structural Analysis

The TNT® SP6 High-Yield Protein Expression System uses a high-yield wheat germ extract supplemented with SP6 RNA polymerase and other components. Coupling transcriptional and translational activities eliminates the inconvenience of separate in vitro transcription and purification steps for the mRNA, while maintaining the high levels of protein expression. All that is required is the addition […]

Optimized Wheat Germ Extract for High-Yield Protein Expression of Functional, Soluble Protein

Cell-free protein synthesis has emerged as powerful alternative to cell based protein expression for functional and structural proteomics. The TNT® SP6 High-Yield Protein Expression System uses a high-yield wheat germ extract supplemented with SP6 RNA polymerase and other components. Coupling transcriptionaland translational activities eliminates the inconvenience of separate in vitro transcription and purification steps for […]