During the week of March 26, 2018, while many students were having fun and relaxing during Spring Break, others were busy doing extra lab work at the BTC Institute. This four-day workshop was designed to provide an introduction to the molecular biology laboratory for students affiliated with the Center for Educational Opportunity (CeO) on the UW Madison campus. As noted on its web site: “CeO promotes access to resources, academic achievement and personal growth for students whose parents have not received a four-year degree, students who meet specific federal family income guidelines, and students with documented disabilities.”
It is well known that first-generation college students, women and students of color persist in STEM fields at lower rates than the general population. This interferes with the creation of a diverse STEM talent pool, in turn needed to ensure diverse problem-solving perspectives.
Further, STEM fields are often seen as being stressful, given their competitive learning environments. This may be especially discouraging for students from racial/ethnic minorities who may not have as many mentors and role models to turn to.
Introduction to the Laboratory attendees
This workshop aimed to give students an experience that would strengthen their skills and confidence as they continue to pursue scientific paths. In addition to laboratory work, students discussed the importance of clear communication in written and oral presentations, were required to work as partners to experience teamwork, and were encouraged to use reflection and lab reporting as ways to internalize what they learned throughout the week. Continue reading →
Pearl Jam, a popular alternative rock band in the 1990s (and still pretty awesome!). Photo credit: Rolling Stone Magazine.
This post could easily start out as an ode to ’90s alternative music (of which I’m a huge fan). That new and totally different sound (a la Pearl Jam, Smashing Pumpkins, Soundgarden, Nirvana, etc.) in the 1990s eventually made its way into the mainstream as it gained popularity. (I have to say that I got a shock when I recently heard some Pearl Jam on “classic rock” radio stations. But I digress…)
Why isn’t the same true for science career paths? Science careers outside of academia are still referred to as “alternative.” Continue reading →
Isabel Agasie speaks with middle school students at FutureQuest 17.
The Dane County School Consortium and the Madison Metropolitan School District’s Career and Technical Education Division collaborated to offer FutureQuest17 on December 6th at the Alliant Energy Center. Designed as a hands-on experience for Dane County middle school students to explore areas of potential interest within a 16 career cluster, over 70 companies provided information and activities for 5300+ attendees.
BTC Institute staff members (Isabel Agasie, Amy Prevost and Karin Borgh) and volunteer Promega production scientists (Molly Nyholm and Kay Rashka) created a lively table area that focused on bioluminescence. Our space included opportunities to see an illustration of the range of careers in a biotechnology company like Promega, practice with different sizes of pipettes, view glowing recombinant luciferase, watch a scrolling slide show illustrating bioluminescence both in nature and in the lab and consider why a scientist might be interested in bioluminescence as a research tool.
Most importantly, we were able to engage in many wonderful conversations, and for this we needed all five of us since the schedule for the day included 14 periods of 20 minutes each—our estimate is that we were able to speak with ~40–50 students during each of these cycles!
As Molly noted:
The questions students asked were fantastic!! “What is the chemical composition of this luciferin solution?” “How much money do you make?” “Do all glowing creatures have the same luciferase enzyme or are they different?” “Are there any bioluminescent fish in Wisconsin?” “Do I have to go to school for as long as you did if I want to be a scientist?” “What pH is this solution?” “Does this have potassium or sodium iodide?” “Can I do an internship?” “Can I be on the culinary team at Promega?” “Does my glow paint have luciferase in it?” “Do you have to take luciferase and luciferin out of those creatures or is there a way to make it in the lab?”
Kay Rashka works with students at FutureQuest17.
And, Isabel added:
It was really great to connect with students and also with teachers. Lots of fun being surrounded by kids and fantastic adults. Some kids were surprised to learn that a biotechnology company hires people in other areas besides science. They asked about diversity and were very glad to hear that there are many different kinds of jobs in biotech companies.
Some of the other presenters in the STEM area of the event that we were in close proximity to included: the City of Madison Engineering Division (where students could construct marble runs that represented water flow), Saris (where students could ride bikes set up to display a training program), Laser Tag (try it out!), very active construction companies’ hammering stations and the MG&E’s electric car. In other words, the level of activity was high, and it was wonderful to contribute to this event—we’ll be back next year!
A few days ago, while taking an unplanned distraction break on Facebook, I came across a video of an enormous coconut crab attacking a red-footed booby. The footage was captured by a biologist studying crab behavior in the Chagos Archipelago in the middle of the Indian Ocean. On this trip he had already confirmed that the monstrous crustaceans snacked on large rats, but he never expected to watch one devour a full bird.
This video sent me on a research journey into other interesting meals discovered by animal researchers. Besides providing sensational headlines about what’s eating what, these studies help us understand everything from nutrient exchange to learned behavior. I’ve compiled a short list of observations and discoveries made in the past few months where researchers have used weird meals to understand complex phenomena. Warning: this might get gruesome! Continue reading →
Amani working in the laboratory of Dr. McFall-Ngai’s as a high school Youth Apprentice
Amani Gillette, a junior from LaFollette High School in Madison, started the Biotechnology Youth Apprenticeship Program (YAP) in Fall Semester, 2010. An outstanding youth apprentice (YA) throughout her two years in the program, she excelled in both the specialized laboratory course at the BTC Institute and in her work site research under the mentorship of Professor Margaret McFall-Ngai, UW-Madison Department of Medical Microbiology & Immunology. Amani’s characterization of a gene and protein found in a small tropical squid resulted in her first scientific publication and poster presentation.
Fast forward— after receiving a B.S. in Biomedical Engineering at Michigan Technological University (which included working in a tissue engineering lab and two summers interning at Promega Corporation under the supervision of Dr. Dan Lazar to help develop an assay for autophagy), Amani is now back in Madison. She is in her second year of graduate school and, working with Dr. Melissa Skala at the Morgridge Institute for Research, is currently mentoring Biotechnology YA Ava VanDommelen (senior from DeForest High School). Following in Amani’s footsteps, Ava will present her research nationally this January at the SPIE conference (the International Society of Optics and Photonics). Continue reading →
A long time ago, before the rise of humans, before the first single celled organisms, before the planet even accumulated atmospheric oxygen, Earth was already turning, creating a 24-hour day-night cycle. It’s no surprise, then, that most living things reflect this cycle in their behavior. Certain plants close their leaves at night, others bloom exclusively at certain times of day. Roosters cock-a-doodle-doo every morning, and I’m drowsy by 9:00 pm every night. These behaviors roughly align with the daylight cycles, but internally they are governed by a set of highly conserved molecular circadian rhythms.
Jeffrey Hall, Michael Rosbash and Michael Young were awarded the 2017 Nobel Prize in Physiology/Medicine for their discoveries relating to molecular circadian rhythms. The official statement from the Nobel Committee reads, “…this year’s Nobel laureates isolated a gene that controls the normal daily biological rhythm. They showed that this gene encodes a protein that accumulates in the cell during the night, and is then degraded during the day. [They exposed] the mechanism governing the self-sustaining clockwork inside the cell.” What, then, does this self-sustaining clockwork look like? And how does it affect our daily lives (1)?
I am a podcast junkie. In a given week I will listen to 15-20 podcast episodes, while only watching a couple television shows. Podcasts allow me to partake in my favorite pastime, learning, while offering distraction from mundane and time-consuming activities.
Podcasts help me pass the time during my daily 1.5+ hour round trip commute, while running (including during races) and in waiting rooms or airport terminals. Not surprisingly, many of these include science podcasts.
So, I was ecstatic to hear about a new science podcast for kids, Wow in the World, that I could share with my 5-year-old daughter. I considered it an experiment, assuming that she would listen to one or two episodes and lose interest, not expecting her to stay engaged by 20 minutes of audio alone.
I couldn’t have been more wrong. Within a few seconds, she was singing along with the theme song and after a couple minutes she was fully engaged and asking questions about what was being discussed. In a world where our DVR is filled with a backlog of recorded shows for her to watch on TV, she had trouble understanding that we had to wait until next week for another episode. In the meantime, she enthusiastically listened to the same episode 3 or 4 times, picking up something new each time.
This year’s participants in Emerging Techniques in Protein and Genetic Engineering, a two-credit graduate course offered in partnership with the Department of Oncology, UW-Madison, held July 17-21, 2017.
Today’s author extends thanks to Heather Gerard, Intellectual Property Manager, Promega Corporation for contributing her expertise to this post.
Students most often come to the BTC Institute with the primary goal of learning about molecular biology technologies. Our mission is to help them update their experimental tool-box, facilitating more capable studies of DNA, RNA and proteins back in their home laboratories.
It is summer here in Wisconsin and the kids are out of school. If you are like me, you are looking for things to keep them busy and (bonus!) maybe teach them something. Below is a list of relatively easy, do-at-home science projects that can be fun for the whole family to try.
Parental supervision is recommended/required for these. And if you don’t want to worry about major clean up (or repainting walls and ceilings) you might want to do these outside whenever possible. I might be speaking from personal experience on this point, so trust me.
Recently a FaceBook friend of mine (who is not a scientist) shared a video from WIRED Science where the concept of CRISPR is explained at 5 Levels of Difficulty— for a 7 year old, a teenager, a college student, a grad student and a CRISPR expert.
First it was pretty amazing to me that my non-scientist friends are interested enough in learning about CRISPR to share this type of information—perhaps showing just how popular and exciting the method has become. People outside the scientific field are hearing a lot about it, and are curious to know more.
This video does a great job of explaining the technique for all its intended audiences. It also is a nice illustration of how to share information in an easily understandable format. Even with the 7 year old and 14 year old, the information is shared in a conversational way, with everyone involved contributing to sharing information about CRISPR.