CRISPR/Cas9 Knock-In Tagging: Simplifying the Study of Endogenous Biology

Understanding the expression, function and dynamics of proteins in their native environment is a fundamental goal thatโ€™s common to diverse aspects of molecular and cell biology. To study a protein, it must first be labeledโ€”either directly or indirectlyโ€”with a โ€œtagโ€ that allows specific and sensitive detection.

Using a labeled antibody to the protein of interest is a common method to study native proteins. However, antibody-based assays, such as ELISAs and Western blots, are not suitable for use in live cells. These techniques are also limited by throughput and sensitivity. Further, suitable antibodies may not be available for the target protein of interest.

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Bringing Science to Life: How Art and Sustainability Shape Our New Trade Show Booth Design

Step inside a Promega booth and leave the ordinary behind. Here, science sparks creativity, sustainability is woven into every detail, and discovery isn’t just something you seeโ€”it’s something you feel.

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The Central Dogma of Promega: The Story and Science Behind Our Kit Packaging Design

An amazing transformation is taking place, unseen and unnoticed, within the microscopic bits that make you, you.

A tightly coiled lattice unspools to reveal a sinuous DNA stand. Along its length, tendrils of RNA sprout, growing bit by genetic bit. Eventually, the signal to stop and break away arrives, yielding a new strand of RNA that faithfully transcribes the DNA strand’s genetic code. Proteins trim and splice this new growth, pruning it so it takes its final form, messenger RNA. More proteins then ferry this mRNA strand through a pore in the nuclear envelope into the open space of the cellโ€™s cytoplasm. Ribosomes and codon-carrying tRNA alight onto the released mRNA strand, reading the instructions it has carried from the DNA in the nuclear nursery. From this trio new forms emerge, bulbous proteins shaped by their destined purpose.

And so it goes, every second of every day, in the tens of trillions of cells in your bodyโ€ฆ

โ€ฆAnd on the tens of thousands of kit packages we deliver to customers across the globe every year.

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Celebrating 10 Years of Innovation: Introducing the CSCB-Promega Innovation Fundย 

For the past decade, Promega has supported cutting-edge research through the CSCB-Promega Innovation Award. Now, as we mark 10 years of collaboration with the Chinese Society for Cell Biology (CSCB), the Award is being converted into the CSCB-Promega Innovation Fund.  

The CSCB, founded in 1980, is one of the leading scientific organizations in China. Dedicated to fostering educational opportunities and innovation, the CSCB organizes conferences, publishes journals, promotes research collaborations, offers training for students and young scientists, and educates the general public on cell biology and biomedicine. 

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Blending Art and Science in a Costa Rica Physics Lab

Sophia Speece engaged her passions for art and science during her internship in Costa Rica.
UW-Madison student Sophia Speece (left) spent the summer in Costa Rica for the “Artist in the Science Lab” internship hosted by alum Dr. Mariela Porras Chaverri (right)

My name is Sophia Speece. I am a junior at the University of Wisconsin-Madison studying Biomedical Engineering and Music Performance. As you can imagine, there is not a lot of overlap between these two passions of mine.

This past summer I was given the unique opportunity to combine these two areas. I applied and was accepted for the โ€œArtist in the Science Labโ€ internship abroad in Costa Rica!

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Insights from our Second Annual Targeted Protein Degradation Symposium

In the opening remarks of our second annual Targeted Protein Degradation Symposium, Tom Livelli, VP of Life Sciences Products & Services at Promega, posed a question to the attendees: โ€œWhat do you want to be able to do today that you canโ€™t?โ€ This aspirational question set the tone for an event where building connections to advance the study and application of proximity-induced degradation took center stage.

More than 90 attendees from academia and industry gathered September 20โ€“21 for the two-day symposium, which was hosted in our inspirational Kornberg Centerโ€”the R&D heart of Promega. Through engaging talks, a poster session, โ€œLearn nโ€™ Burnโ€ challenges and social gatherings, participants had the opportunity to reinforce existing collaborations and to connect with others who are making an impact in the field of targeted protein degradation.

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Promega Fall Art Showcase Honors Truman Lowe, Ho-Chunk Artist and Mentor

Truman Lowe, Ho-Chunk artist and mentor, pictured with Headdress (1989)

Outside the BioPharmaceutical Technology Center, the wind snakes through the tall prairie grasses, drying slowly in the crisp September air. The walking paths through the woods are turning orange with fallen leaves, and the resident sandhill cranes, a fixture of summer at Promega Madison, will soon be heading to their winter home in southern Florida.

Inside the BTC, the Promega Fall Art Showcase is honoring the life of Truman Lowe, an acclaimed Ho-Chunk artist whose sculptural works evoke a powerful connection with nature. For decades, Lowe was a professor in the Department of Art at the University of Wisconsin-Madison where he was an exceptional mentor to young artists. He was known for encouraging artists to fearlessly delve into their artistic voice and equipping them with the necessary tools to navigate the art world. The Fall Art Showcase honors his legacy by exhibiting his art alongside pieces by several former students.

Truman Lowe: Visionary Artist, Mentor and Teacher

Truman Lowe was born on the Ho-Chunk Nation of Wisconsin reservation in 1944. He recounted a childhood of drawing with rocks on the Black River and creating crafts like baskets and beadwork with his parents. Though he loved art from an early age, Lowe says that he never thought of art as a profession until he was studying for his undergraduate art degree at the University of Wisconsin-LaCrosse. There, he became fascinated with Michelangelo, who inspired him to realize that art could be โ€œa profession as well as a passion.โ€1

Visitors to the Promega Fall Art Show can see sculptures by Truman Lowe alongside works by several of his students.

After earning a graduate degree and moving through several teaching positions at the high school and university level, Lowe accepted a position as Native American studies coordinator and assistant professor of art at UW-Madison in 1975. This began a 45-year tenure in the department of art, where he fostered a deep understanding and appreciation for Native American art and culture among his students. He also served as a curator of contemporary art at the National Museum of the American Indian.

Lowe is known for large, site-specific installations that use natural materials including wood, stone and metal. His works push creative boundaries and exhibit a unique blend of versatility, precision and emotional depth. Loweโ€™s sculptures have been exhibited around the world, from museums including the Metropolitan Museum of Art to embassies in Bolivia and Cameroon. A sculpture titled Effigy: Bird Form was displayed on the White House grounds during the Clinton administration and was recently reinstalled atop Observatory Hill at UW-Madison, close to the former site of Native American effigy mounds. 

Lowe, who died in 2019, was a beloved mentor to many students over his long tenure at UW-Madison. A university-published obituary quotes John Hitchcock, professor and Associate Dean at UW-Madison, saying, โ€œTruman encouraged us to stay strong as artists and to our vision as makers.โ€ The widespread love of Lowe will be on display at the Promega Fall Art Showcase, where six of his former students will be exhibiting alongside Loweโ€™s own works.

Promega Fall Art Showcase

The Fall Art Showcase opened on September 19 with a symposium featuring guest speakers Patricia marroquin Norby and Jo Ortel. Norby is the Associate Curator of Native American Art at the metropolitan Museum of Art, and the first person of Indigenous descent hired for a full-time curatorial position in the museumโ€™s 150-year history. Jo Ortel is an author, art historian and Professor Emerita of Art History of Beloit College. Ortel is also the author of a notable biography of Truman Lowe titled โ€œWoodland Reflections: The Art of Truman Lowe.โ€

The Promega Culinary Team collaborated with Chef Elena Terry of the culinary organization Wild Bearies to offer traditional Ho-Chunk food at the reception. Chef Terry provided recipes and connected the team with indigenous purveyors to source ingredients. Promega also collaborated with Little Eagle Arts Foundation to include pieces of Ho-Chunk culture into the event.

The Fall Art Showcase runs through December 29 and is open to the public Monday through Friday 8:00 am โ€“ 4:00 pm at the Promega BioPharmaceutical Technology Center. For more information, visit https://www.promega-artshow.com/

1As quoted in Woodland Reflections: The Art of Truman Lowe by Jo Ortel


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Promega qPCR Grant Series #1: Marine Plant Ecologist, Dr. Agustรญn Moreira-Saporiti

Dr. Agustรญn Moreira-Saporiti is a postdoctoral researcher at the Marine Biological Laboratory and is studying flowering processes in marine seagrass

Marine seagrasses are submerged flowering plants that form essential underwater meadows, fostering diverse ecosystems and providing a habitat for marine life. Our first Promega qPCR Grant winner and marine ecologist, Dr. Agustรญn Moreira-Saporiti, plans to continue adding to a fascinating body of work aimed at understanding flowering in marine seagrasses.

Dr. Moreira-Saporiti began his journey into marine plant ecology at the University of Vigo, Spain, where he earned a bachelorโ€™s degree in marine sciences. He then went on to complete a masterโ€™s degree at the University of Bremen (Germany) where his thesis focused the ecology of seagrasses in Zanzibar, Tanzania. His passion for marine botany led him down a deeper exploration of marine plants, unraveling the intricate web of ecosystem processes within seagrasses.

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2023 Promega iGEM Grant Winners: Tackling Global Problems with Synthetic Biology Solutions

On June 15, 2023, we announced the winners of the 2023 Promega iGEM grant. Sixty-five teams submitted applications prior to the deadline with projects ranging from creating a biosensor to detect water pollution to solving limitations for CAR-T therapy in solid tumors. The teams are asking tough questions and providing thoughtful answers as they work to tackle global problems with synthetic biology solutions. Unfortunately, we could only award nine grants. Below are summaries of the problems this year’s Promega grant winners are addressing.

UCSC iGEM

An immature night heron against the green surface of Pinto Lake. 2023 Promega iGEM Grant Winner, UCSC iGEM seeks to mitigate these harmful aglal blooms.
A night heron hunts on Pinto Lake, California.

The UCSC iGEM team from the University of Californiaโ€“Santa Cruz is seeking a solution to mitigate the harmful algal blooms caused by Microcystis aeruginosa in Pinto Lake, which is located in the center of a disadvantaged community and is a water source for crop irrigation. By engineering an organism to produce microcystin degrading enzymes found in certain Sphingopyxis bacteria, the goal is to reduce microcystin toxin levels in the water. The project involves isolating the genes of interest, testing their efficacy in E. coli, evaluating enzyme production and product degradation, and ultimately transforming all three genes into a single organism. The approach of in-situ enzyme production offers a potential solution without introducing modified organisms into the environment, as the enzymes naturally degrade over time.

IISc-Bengaluru

Endometriosis is a condition that affects roughly 190 million (10%) women of reproductive age worldwide. Currently, there is no treatment for endometriosis except surgery and hormonal therapy, and both approaches have limitations. The IISc-Bengaluru team at the Indian Institute of Science, Bengaluru, India, received 2023 Promega iGEM grant support to investigate the inflammatory nature of endometriosis by targeting IL-8 (interleukin-8) a cytokine. Research by other groups has snow that targeting IL-8 can reduce endometriotic tissue. This team will be attempting to create an mRNA vaccine to introduce mRNA for antibody against IL-8 into affected tissue. The team is devising a new delivery mechanism using aptides to maximize the delivery of the vaccine to the affected tissues.

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From Student Debt Assistance to Family-Forming Support: Benefits and Well-Being Go Hand in Handย 

You know this, but it bears repeating. Prioritizing physical, psychological, emotional and financial wellness is key to supporting our overall well-being. This holds true during the busy holiday season and throughout the entire year.  

Employee benefits that address emotional, physical and financial well-being

It can also be easier said than done, so we need as much support as possible in this vital endeavor. An in-depth framework recently published by the US Surgeon General outlines a vision of the workplace as an engine of well-being. The report states, โ€œOur workplaces play a significant role in our lives. Work affects both our physical and mental well-being โ€“ in good ways and bad. The COVID-19 pandemic brought the relationship between work and well-being into clearer focus.โ€   

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