Many research labs around the world have temporarily closed their doors in response to the COVID-19 pandemic, while others are experiencing unprecedented need for reagents to perform viral testing. This urgency has led many scientists to make new connections and build creative, collaborative solutions.
“In labs that are still open for testing or other purposes, there’s certainly heightened anxiety,” says Tony Vanden Bush, Client Support Specialist. “I feel that right now, I need to help them deal with that stress however possible.”
Last week, Tony was contacted by a lab at the University of Minnesota that was preparing to serve as a secondary COVID-19 testing facility for a nearby hospital lab. The two labs needed to process up to 6,000 samples per day, and the university lab was far short of that capacity.
This blog was written with much guidance from Jennifer Romanin, Senior Director IVD Operations and Global Service and Support, and Ron Wheeler, Senior Director, Quality Assurance and Regulatory Affairs at Promega.
A Trip Down Memory Lane
Back in the day when we all walked two miles uphill in the snow to get to our laboratories, RNA and DNA extraction was a home-brew experience. You made your own buffers, prepped your own columns and spent hours lysing cells, centrifuging samples, and collecting that fluorescing, ethidium bromide-stained band of RNA in the dark room from a tube suspended over a UV box. Just like master beer brewers tweak their protocols to produce better brews, you could tweak your methodology and become a “master isolater” of RNA. You might get mostly consistent results, but there was no guarantee that your protocol would work as well in the hands of a novice.
Enter the biotechnology companies with RNA and DNA isolation kits—kits and columns manufactured under highly controlled conditions delivering higher quality and reproducibility than your home-brew method. These systems have enabled us to design ever more sensitive downstream assays–assays that rely on high-quality input DNA and RNA, like RT-qPCR assays that can detect the presence of a specific RNA molecule on a swab containing only a few hundred cells. With these assays, contaminants from a home-brew isolation could result in false positives or false negatives or simply confused results. Reagents manufactured with pre-approved standard protocols in a highly controlled environment are critical for ultra sensitive tests and assays like the ones used to detect SARS-CoV-2 (the virus that causes COVID-19).
The Science of Manufacturing Tools for Scientists
There are several criteria that must be met if you are
producing systems that will be sent to different laboratories, used by
different people with variable skill sets, yet yield results that can be
compared from lab to lab.
As scientists, we often find ourselves fielding questions about events in the news that may or may not be related to our area of expertise. Especially during the ongoing pandemic, it can often be difficult to share accurate information without either sparking panic or understating the severity. Nonetheless, we want to support our friends and family in times of uncertainty, and one way to do that is by sharing accurate information about scientific topics.
We’ve gathered answers to a few frequently asked questions
about the COVID-19 pandemic that we’ve received from family members. Have
question we missed? Submit it in the comments and we’ll get back to you.
I was confident I knew a few things about the bubonic plague: It was caused by the bacterium Yersinia pestis, which was transmitted to humans by fleas hitching a ride on the back of traveling rats. It spread rapidly and devastated populations around the globe, and because cats, a natural predator of scurrying rodents, had been killed, rats proliferated along with their deadly, infectious cargo. However, until I read a recent PLoS ONE article, I did not realize there was still debate about whether Yersinia pestis was the infectious agent for Black Death, the disease that ravaged 14th century Europe and killed one third of its population. Continue reading “What Caused the Black Death?”
It’s hard not to panic in the light of recent pandemic fears and the frightening possibilities conjured up by the thought of a novel flu virus with the propensity for person-to-person spread (1-3). The specter of the 1918 pandemic has raised its ugly head, and we are left feeling intensely vulnerable to an invisible and ever-changing enemy. Have science and history left us more prepared to combat this virus than those who suffered during the devastating 1918 outbreak? Continue reading “H1N1 Influenza”