Can We Have Healthy Bees and Green Lawns?

bumble bee pollinating golden rod at the Promega Madison campus
Bumblebee on the prairie at Promega headquarters in Madison, WI. Copyright Promega Corporation.

Some people like a perfectly green carpet of grass; I welcome the biodiversity of clover, dandelions and other weeds (although I could do without the painful thistles). Of course, I also notice many pollinating insects including bumblebees seem to enjoy visiting the flowers that bloom in the lawn. However, concerns have arisen over the use of insecticides and the health of our pollinators. There has been extensive talk about the collapse of honeybee colonies and declining populations of native pollinators like bumblebees. One area of research is the use of insecticides, especially neonicotinoids, and how they affect bees but most studies were done in lab environments, not in a lawn where the bees would collect nectar from flowering plants. Larson, Redmond and Potter decided to study both clothianidin, a neonicotinoid insecticide, and chlorantraniliprole, an anthranilic diamide, and examine how the insecticides affected bumblebees that foraged on treated lawns.

Neonicotinoids are systemic insecticides that are potent selective agonists of nicotinic acetylcholine receptors in insects, and are applied as sprays or granules during the spring for control of root-feeding grubs and other pests. Anthranilic diamides are a new class of insecticides that activate insect ryanodine (calcium channel) receptors and cause lethal paralysis in sensitive species. Chlorantraniliprole shows low acute bee toxicity, a promising development, but this anthranilic diamide has not been tested in the field.

Continue reading “Can We Have Healthy Bees and Green Lawns?”

The Do’s and Don’t’s of Resume Writing

interviewAs a recruiter, I look at resumes every single day. It’s part of my job. A good resume will get your information passed on to a hiring manager, and a bad one will end up in a rejection pile. A candidate could be a perfect fit for the job, but if the resume does not reflect HOW they are a perfect fit, they will not get moved forward in the interview process.

I write this blog with the caveat that I am not the end-all, be-all for resumes. You can do research and find many different ways to write a resume, advice on what to do, and examples galore. I am writing this with my preference in mind; I have been a recruiter for over a decade and have seen more resumes that you can imagine. I find that these basic do’s and don’ts apply to many different professions.

DO

  1. Change your resume based upon the position that you are applying for. If you have an objective, the objective should somehow tie into the position you are submitting your resume to. If your objective is very broad and can apply to many different areas, that should be fine. However, if your objective reads “To find employment as a Research and Development Scientist” and you are applying for a Production Scientist position, you should change it.
  2. List your work history in reverse chronological order. Your most current job should be at the top.
  3. If you are applying for a position where education is the most important thing to consider for the recruiter/hiring manager, then list it first. This will also apply to a recent graduate. For any other type of resume submission, put your education below your work experience.
  4. Be very specific in your accomplishments. This is your opportunity to brag about your work experience. If you were the #1 sales person in the country, list it on your resume.
  5. Be quantitative. Specifically list numbers in your descriptions. “Increased sales by 10%”, or “Managed a team of 5” sounds a lot better than “Increased sales substantially” or “Managed a team”.

DON’TS

  1. Don’t try to fit it into one page. This is an old guideline that is somehow still being used. If you can’t fit it into one page, don’t try. You want your resume to outline as much as possible about your work experience, so don’t try to limit to fit it all in.
  2. Don’t make it 10 pages either. Recruiters (and most hiring managers) will stop looking after about page 2. Keep it concise.
  3. Don’t write in paragraphs. Write concise statements (bullets are good) with duties and responsibilities at jobs.
  4. Don’t list all the things that you have done in your previous positions at the top of your resume, and then list your work history below it. Your duties and responsibilities should coincide with the places you have worked.
  5. Don’t forget to proofread!

Healthy Lifestyles: Good for You and Your Telomeres Too

DNA in a test tubeWe all know that a healthy lifestyle (diet high in whole foods and low in fat, moderate exercise, managing stress and good social support) is good for us. In fact I will go so far as to say that it isn’t even news that these things help our health and well-being.  What is news, or at least newly published, is that these changes may also have a positive effect on telomerase activity and telomere length (1). Continue reading “Healthy Lifestyles: Good for You and Your Telomeres Too”

Is This What a Scientist Looks Like?

scientists-at-workI am the mother of a six-year-old girl who loves to get magazines in the mail. For several years my daughter has received an enjoyed popular kids’ science/international culture magazine. The stories are short and simple, and this magazine usually does a good job of presenting factual information in easy-to-digest forms. Each magazine comes with a set of animal cards, which we have diligently collected.

However, the latest issue that came to our mailbox really got me thinking. The final pages featured artwork by the young readers. I love the idea of featuring the work of the readers.  Usually, my daughter loves seeing what other children her age from around the world draw and take pictures of, and sometimes we have some pretty interesting discussions about the work.

This time though we didn’t spend much time talking about the art work. She wasn’t particularly interested, and I wasn’t sure I what I thought. But I may have missed a teachable moment. The theme for the pages was a Halloween-minded “spooky science”, and all of the pictures were of “mad scientists” alone at work doing presumably nefarious things in their laboratories. Of the eight drawings pictured, six of them pictured scientists that were human, and five of the humans were male. All of them were pale-skinned. The sole female scientist, whose lab featured a certificate with the words “monster maker”, was drawn by a girl. The ages of the children submitting the work ranged from 9 to 14. Continue reading “Is This What a Scientist Looks Like?”

Trypsin/Lys-C Mix: Alternative for standard trypsin protein digestions

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.It is a little known fact that trypsin cleaves at lysine residues with lesser efficiency than at arginine residues. Inefficient proteolysis at lysine residues is the major cause of missed (undigested) cleavages in trypsin digests.

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Supplementing trypsin with Lys-C enables cleavage at lysines with excepetional efficiency and specificity. Following the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions, reduction,alkylation, 25:1 protein:protease ratio [w/w], mix and incubate overnight at 37°C.) Replacing trypsin with Trypsin/Lys-C Mix in this conventional protocol leads to multiple benefits for protein analysis including more accurate mass spectrometry-based protein quantitation and improved protein mass spectrometry analytical reproducibility.

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Extreme Frog Calling: More Effort Equals Greater Interest

Hyla versicolor (Copes grey treefrog) Photo credit: LA Dawson wikipedia
Hyla versicolor (Copes grey treefrog) Photo credit: LA Dawson wikipedia

Ever since a colleague introduced me to the Spadefoot Toad and the practice of monitoring frog and toad calls during the summer months as one way of tracking the prevalence and health of frogs and toad populations over time, I have been intrigued by any research on frog and toad calling. So, when I saw news headlines about “multitasking” Copes grey treefrogs (Hyla chrysocelis) and their “popularity” among the females of their species, I couldn’t resist.

The paper, published in Animal Behaviour by Ward and colleagues describes an investigation of the predictions of the “multitasking hypothesis”. Briefly this hypothesis predicts two things. The first prediction states that when you have a signal that has components that negatively covary (in order for one component to occur the second one is reduced), say pitch and volume, there will be a tradeoff between the two things (e.g., I can sing at a really high pitch, but not very loudly). The second prediction is that the individual on the receiving end of this signal is going to prefer the individual who can do both at the same time (e.g., sing at a really high pitch, very loudly). In this study, the authors looked at female frog choice based on three factors: call rate (the number of calls per minute), call duration (number of pulses per call) and call effort (calls/min x pulses/call = pulses/min).  They asked two questions: Is there a tradeoff between rate and duration, and do female frogs prefer the male frogs that exhibit the higher overall “call efforts”?

Continue reading “Extreme Frog Calling: More Effort Equals Greater Interest”

What Came First: LP or the Cow? Genetic and Cultural Co-evolution of Lactase Persistence and Dairying

Cow with milk.The ability for adults to digest the milk sugar, lactose, is often referred to as lactase persistence (LP), describing the continued (persistent) production of lactase into adulthood. LP is an autosomal dominant trait that is most often associated with a T allele situated 13,910 base pairs (–13,910*T allele) upstream of the lactase gene, LCT. Archaeogenetic data indicates that pre- and early Neolithic populations were largely LP-negative, and that the frequency of the LP phenotype rose dramatically in Europe around 8,000 years ago, coinciding with the Neolithic transition from a hunter-gather to an agricultural-based lifestyle (1) and the appearance of domesticated dairying animals. Today roughly 35% of adults are lactose persistent. The frequency varies dramatically by geographic region, from a high prevalence in Europe (89–90%) and to a relatively low prevalence in the eastern Mediterranean (15%)(1). The spread of lactase persistence is an often cited example of gene-culture co-evolution. You can’t separate the history of domestic dairying and the evolution of lactase persistence, but scientists are still trying to understand how these two worked together. Continue reading “What Came First: LP or the Cow? Genetic and Cultural Co-evolution of Lactase Persistence and Dairying”

Elevating “bliss” the natural way

Sun RunnersMarijuana is a highly controversial substance with roughly an equal number of supporters and opponents of its use for medicinal purposes. Marijuana is a dry, shredded mix of flowers, stems, seeds and leaves of the Hemp plant Cannabis sativa. New studies reporting the efficacy of medicinal marijuana in clinical conditions surface on a fairly regular basis, with the latest being a reported treatment for seizures. This constant influx of new information shows how little we know about the substance and how it works in the human body. So what do we know about this substance? While many psychoactive drugs clearly fall into the category of either stimulant, depressant or hallucinogen, Cannabis exhibits a mix of all properties, perhaps leaning the most towards hallucinogenic or psychedelic, though with other effects quite pronounced as well. Continue reading “Elevating “bliss” the natural way”

Preventing the Heartache of Cell Line Misidentification

Golden mask

It’s a scientist’s nightmare: Spending time and resources to investigate a biological phenomenon only to learn later that your cells are not what you think they are—their true identities hidden. As a result, all of the data that you’ve generated with those cells, published and unpublished, are cast into doubt. You thought that you knew your cells, that you could trust them, but your trust was misplaced. At some point, perhaps even before the traitorous cell line entered your laboratory, the cells were mislabeled, misidentified or contaminated with another cell line. It didn’t have to be this way. There are easy steps you can take to prevent the headache and heartache of cell line misidentification and contamination.

Continue reading “Preventing the Heartache of Cell Line Misidentification”

Site-specific copy number variations in cancer: A story begins to unfold

Designed by Nick Klein for ISO-form, courtesy of Promega.
Designed by Nick Klein for ISO-form, courtesy of Promega.

Tumor cells are characterized by many features: including uncontrolled proliferation, to loss of contact inhibition, acquired chromosomal instability and gene copy number changes among them. Some of those copy number changes are site-specific, but very little is known about the mechanisms or proteins involved in creating site-specific copy number changes. In a recently published Cell paper, Black and colleagues, propose a mechanism for site-specific copy number variations involving histone methylation proteins and replication complexes.

Previous work from Klang et al. had shown that local amplification of chromosomal regions occurs during S phase and that chromatin structure plays a critical role in this amplification (2), and other work by Black and colleagues (3) implicated KDM4A in changing timing of replication by altering chromatin accessibility in specific regions. Other research also had shown that KDM4A protein levels influence replication initiation and that KDM4A has a role in some DNA damage response pathways (4,5).  Looking at the body of work, Black et al. hypothesized that KDM4A, with its roles in replication, might possibly provide link into the mechanism of site-specific copy number variation in cancer. Continue reading “Site-specific copy number variations in cancer: A story begins to unfold”