This guest blog post is written by Tara Luther, Sr. Marketing Specialist, Genetic Identityat Promega.
April 2024 marked the 23rd anniversary of Sexual Assault Awareness Month (SAAM) in the United States. While we have crossed into May, the importance of what SAAM stands for does not diminish with the changing of the calendar. The National Sexual Violence Resource Center (NSVRC) emphasizes that SAAM is a time to not only draw attention to the prevalence of sexual assault, but to also educate individuals and communities about how to prevent sexual violence.
An amazing transformation is taking place, unseen and unnoticed, within the microscopic bits that make you, you.
A tightly coiled lattice unspools to reveal a sinuous DNA stand. Along its length, tendrils of RNA sprout, growing bit by genetic bit. Eventually, the signal to stop and break away arrives, yielding a new strand of RNA that faithfully transcribes the DNA strand’s genetic code. Proteins trim and splice this new growth, pruning it so it takes its final form, messenger RNA. More proteins then ferry this mRNA strand through a pore in the nuclear envelope into the open space of the cell’s cytoplasm. Ribosomes and codon-carrying tRNA alight onto the released mRNA strand, reading the instructions it has carried from the DNA in the nuclear nursery. From this trio new forms emerge, bulbous proteins shaped by their destined purpose.
And so it goes, every second of every day, in the tens of trillions of cells in your body…
…And on the tens of thousands of kit packages we deliver to customers across the globe every year.
On April 21st, 2024, hundreds of volunteers accompanied four local artists in Madison, Wisconsin for a public painting event to help decorate the 400-600 blocks of State Street. This project is the first embodiment of the city of Madison’s pedestrian mall experiment, set to kick off in May. Taylor McAda, Promega Senior Graphic Designer, was selected to design and paint one of four original 20-foot circle murals.
For decades, pharmaceutical companies have relied on high-throughput screening (HTS) as the first step in the drug discovery process. After an initial screening of thousands of compounds, scientists select a smaller list of candidate drugs that is then used for further downstream testing. A major limitation to HTS, however, is the need to synthesize all compounds used in the screen—the compounds need to physically exist to be tested. This significantly limits the number of compounds that can be tested, hindering the discovery of new drugs.
What if we could test compounds even before they are synthesized?
In the Spring of 2015, greenhouse tomato plants grown in Jordan presented with a mosaic pattern of light and dark green patches on leaves, narrowing leaves, and yellow- and brown-spotted fruit (Salem et al. 2015). The pathogen was identified as a novel plant virus, the tomato brown rugose fruit virus (ToBRFV), and the original outbreak was traced back to the fall of 2014 to Israel (Luria et al. 2017). This newly emerging virus can infect tomato and pepper plants at any stage of development and greatly affect crop yield and quality. Furthermore, the virus spreads rapidly by mechanical contact but can also be spread over long distances by contaminated seeds (Caruso et al. 2022), and as of 2022 it had been detected in 35 countries across four continents (Zhang et al. 2022). Compounding its transmissibility, is the ability of the virus escape plant genetic resistance to viral infection (Zhang et al. 2022). There are seven host plants for the virus, including some common grasses and weeds, which could act as a reservoir for the virus, even if it is eliminated from commercial crops. Some researchers consider ToBRFV to be the most serious threat to tomato production in the world.
Katelyn Geleynse presses the button and the machine groans to life. The massive metal plates shift until a half-ton of compressed plastic tumbles out onto the waiting pallet. The crowd cheers. Permanent markers are passed around and everyone takes turns signing the massive block.
It’s February 16, 2024, and the members of the Promega Sustainability Committee are gathered to witness the first bale of plastic film being ejected from the Madison campus’s new baler. It’s only the first bale, but it represents a major step in the company’s efforts to reduce plastic waste.
Members of the Sustainability Committee gather at Kepler Center to celebrate the first plastic film bale prepared by the new baler.
“All of this would have gone to the landfill if we hadn’t set up this program,” Katelyn says. “It feels good to know that at least some of my waste is getting a second life.”
Plastic film is notoriously difficult to recycle and takes decades to break down in a landfill. The Promega initiative to divert this waste was started by a small group of employees who noticed a problem and worked for over a year to build a sustainable solution. What began as a small volunteer operation grew to spur capital investment in a process that will rescue around 35,000 pounds of plastic per year.
Farmers everywhere strive to protect their crops and ensure a stable food supply while minimizing environmental harm. A promising approach to achieving this leverages a plant’s built-in defense mechanisms, reducing the need for chemical interventions. Many geneticists and agronomists lean on technologies that can automate and streamline nucleic acid extraction and pathogen detection to identify naturally pest resistant crops and, ultimately, keep up with the changing agricultural landscape.
The National Cancer Institute’s NCI-60 drug screening panel, comprised of 60 diverse human cancer cell lines, has been a cornerstone in advancing cancer research and drug discovery since its inception in the late 1980s. Developed in response to the need for more predictive and comprehensive preclinical models, the NCI-60 facilitates the screening of thousands of compounds annually, aiming to identify potential anti-cancer drugs across a broad spectrum of human cancers. This article traces the origins, development, and evolution of the NCI-60 panel, highlighting its significant role in advancing our understanding of cancer and therapeutic agents.
Each year, Promega employees are offered the opportunity to receive up to 40 hours paid time off to donate in volunteer service through our Promega In Action program. Providing sustained support of organizations in our community, our employees participate in a wide range of activities.
In 2023, we awarded 26 individuals who volunteered for 23 different organizations, some bringing along their Promega team members in attendance. From crafting comfort shawls for families of future organ and tissue donors, to volunteering with Meals on Wheels, to journeying to South Africa to deliver charitable donations for children in need, the opportunities to contribute to our community are abundant and impactful.
Your ability to navigate space and time is anchored in your memory, particularly episodic memory, which catalogues the experiences you have in a given location. This type of memory is shaped by complex neural networks firing within your hippocampus. So how exactly do we store memories of the hundreds of things that happen to us in a day, especially when they unfold in the same settings?
There are theories as to how we form single-shot, or “episodic”, memories, many of which center around the activity of place cells, which light up when you are in a specific environment. The idea here is that, with every event that happens in a place, these cells would shift and fire in novel patterns. Scientists at the Zuckerman Mind Brain Behavior Institute at Columbia University questioned this—while it is known that place cell activity can certainly be affected by experiences, they wondered whether there could be an alternative explanation for episodic recall that wouldn’t require the constant remapping of one’s core memory of a place.
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