DNA forensics

How an Innovative Mobile DNA Analysis Lab Helped Identify War Victims in Ukraine

Posted on by Natalie Larsen

Each year, the International Symposium for Human Identification (ISHI) covers a variety of the latest topics in DNA forensics through sessions, workshops and poster presentations. While last year’s meeting largely focused on using investigative genetic genealogy (IGG) and developments in DNA databases, another topic that garnered widespread interest was current efforts being taken to mobilize DNA analysis labs.

One of the speakers, Sylvain Hubac, PhD, Head of the DNA Division of the Forensic Science Laboratory of the French Gendarmerie, helped create a mobile DNA lab solution back in 2015. This innovative mobile lab, designed and patented by the French Armed Forces for the French Gendarmerie (IRCGN), has been used to facilitate DNA identification in a number of emergency settings since it’s inception. From identifying the remains of the Germanwings Flight 9525 plane crash victims in 2015 and the victims of the 2016 Bastille Day terrorist attack in Nice, to adapting the lab to aid in sample processing during the COVID-19 pandemic, the mobile lab has played an especially instrumental role in Disaster Victim Identification (DVI) scenarios.

However, the mobile lab was more recently employed in a new DVI context: identifying victims of the conflict in Ukraine. On the last day of ISHI 33, Dr. Hubac presented on the unique challenges posed when identifying victims of war, and the tools, protocols and system that made the mobile lab uniquely suited for this purpose.

Continue reading “How an Innovative Mobile DNA Analysis Lab Helped Identify War Victims in Ukraine”

Pursuing Justice For Victims: DNA Forensics

Posted on by Riley Bell

Last year’s International Symposium of Human Identification (ISHI) covered a span of topics during various workshops, sessions, and poster presentations. Topics ranged from investigative genetic genealogy (IGG) to customary updates about CODIS and DNA testing standards to mobilizing DNA analysis labs. However, one topic particularly stood out to attendees—Ashley Spence’s powerful testimony on pursuing justice for victims.  

Continue reading “Pursuing Justice For Victims: DNA Forensics”

The 33rd International Symposium on Human Identification: The Past, Present and Future of Investigative Genetic Genealogy

Posted on by Jordan Nutting

It’s hard to imagine a better way to celebrate the 33rd International Symposium of Human Identification than a night spent wandering through the Hall of Human Evolution at the Smithsonian Museum of Natural History. The meeting, which took place in Washington D.C. from October 31–November 4, focused largely on using investigative genetic genealogy (IGG). When used to identify human remains or solve cold cases, IGG (a.k.a. forensic genetic genealogy or forensic investigative genetic genealogy, take your pick) relies heavily on techniques developed to sequence DNA from ancient human remains.

A crowd dances in a stone courtyard with a taxidermy elephant on display.
Credit: ISHI

New to ISHI this year were live-streamed presentations, building off the success of last year’s session recordings for online streaming. Another first was attendees dressing up in costume for the welcome reception, which happened to coincide with Halloween. From a nucleic acid-themed group costume to Sims characters to a bunch of grapes, ISHI 33 attendees had a chance to show off their fun side while reconnecting with colleagues.

While a range of topics were covered during the workshops, sessions and poster presentations, three themes stood out to this first-time ISHI attendee. In addition to IGG, there was widespread interest in developments in DNA databases as well as efforts to mobilize DNA analysis labs.

Continue reading “The 33rd International Symposium on Human Identification: The Past, Present and Future of Investigative Genetic Genealogy”

The 30th International Symposium on Human Identification: Elevating DNA Forensics

Posted on by Ken Doyle

Thirty Years of ISHI

30 years of ISHI

In the fall of 1989, a small group of forensic scientists, law enforcement officials and representatives from Promega Corporation gathered in Madison, Wisconsin, for the very first International Symposium on Human Identification (ISHI). At the time, DNA typing was in its infancy and had not yet been validated as a forensic method. The available technology consisted of two methods: detection of restriction fragment length polymorphisms (RFLPs) and variable number of tandem repeats (VNTRs). Promega had developed products based on both analytical methods, which essentially provide a DNA “fingerprint” or profile for each individual tested.

Among the attendees at that first symposium was Tom Callaghan, then a graduate student. That experience made a significant impact on his career path. Last week, at ISHI 30, he presented a session on rapid DNA testing. Dr. Callaghan currently serves as a Senior Biometric Scientist for the FBI. In 1999, he was instrumental in launching the FBI’s Combined DNA Index System (CODIS) and in 2003, he became the first CODIS Unit Chief.

Continue reading “The 30th International Symposium on Human Identification: Elevating DNA Forensics”

Is MPS right for your forensics lab?

Posted on by Promega

Today’s post was written by guest blogger Anupama Gopalakrishnan, Global Product Manager for the Genetic Identity group at Promega. 

Next-generation sequencing (NGS), or massively parallel sequencing (MPS), is a powerful tool for genomic research. This high-throughput technology is fast and accessible—you can acquire a robust data set from a single run. While NGS systems are widely used in evolutionary biology and genetics, there is a window of opportunity for adoption of this technology in the forensic sciences.

Currently, the gold standard is capillary electrophoresis (CE)-based technologies to analyze short tandem repeats (STR). These systems continue to evolve with increasing sensitivity, robustness and inhibitor tolerance by the introduction of probabilistic genotyping in data analysis—all with a combined goal of extracting maximum identity information from low quantity challenging samples. However, obtaining profiles from these samples and the interpretation of mixture samples continue to pose challenges.

MPS systems enable simultaneous analysis of forensically relevant genetic markers to improve efficiency, capacity and resolution—with the ability to generate results on nearly 10-fold more genetic loci than the current technology. What samples would truly benefit from MPS? Mixture samples, undoubtedly. The benefit of MPS is also exemplified in cases where the samples are highly degraded or the only samples available are teeth, bones and hairs without a follicle. By adding a sequencing component to the allele length component of CE technology, MPS resolves the current greatest challenges in forensic DNA analysis—namely identifying allele sharing between contributors and PCR artifacts, such as stutter. Additionally, single nucleotide polymorphisms in flanking sequence of the repeat sequence can identify additional alleles contributing to discrimination power. For example, sequencing of Y chromosome loci can help distinguish between mixed male samples from the same paternal lineage and therefore, provide valuable information in decoding mixtures that contain more than one male contributor. Also, since MPS technology is not limited by real-estate, all primers in a MPS system can target small loci maximizing the probability of obtaining a usable profile from degraded DNA typical of challenging samples.

Continue reading “Is MPS right for your forensics lab?”

Forensic Scientists Improve Sexual Assault Kit Turnaround Time with Y-Screening

Posted on by Michele Arduengo

The backlog of sexual assault kit samples in crime laboratories across the nation is a topic that hit the spotlight when a group of journalists uncovered the issue in an open records search of crime lab records in 2015. Reasons for the backlog include lack of staff, lack of funding, and simply, lack of time or a decision not to prosecute the case. Processing samples can be a labor-intensive process.

We recently interviewed Lynndsey R. Simon, Forensic Scientist II and Alternate CODIS Administrator from the Columbus Police Forensic Services Center to discuss some recent changes in sample processing in their laboratory that are helping to alleviate some of the backlog. She will be presenting a talk at the upcoming International Symposium on Human Identification (ISHI) in September.

The Columbus Police Forensic Services Center is a smaller forensic laboratory and according to Simon, one of the biggest challenges they face is strained resources. The DNA extraction and processing kits that forensic laboratories use are very expensive, and the number of DNA samples that laboratories are getting for DNA analysis are increasing. With limited resources and funding, maximizing efficiency and finding the best solutions for the laboratory becomes critical. Continue reading “Forensic Scientists Improve Sexual Assault Kit Turnaround Time with Y-Screening”

STR-Validator: Open Source, Free Software for Evaluating Validation Data in the Forensic Laboratory

Posted on by Promega

Before an established method or procedure can be employed in a forensic laboratory, an internal validation must be completed to show that the method performs as expected. Guidelines for validation are outlined by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the European Network of Forensic Science Institutes (ENFSI) DNA Working Group. Validation experiments that meet these guidelines will demonstrate the sensitivity and reliability of a short tandem repeat (STR) typing multiplex system. After a lab completes these validation experiments, it will have sufficient data to determine the analytical and stochastic thresholds of the capillary electrophoresis (CE) instrument in combination with the amplification system, the impact of multiple contributors to a DNA sample and the limit of detection and accuracy of the assay.

Such forensic lab validations are time consuming and can be intimidating, and the requirement to validate new technologies and systems is often seen as a deterrent to the adoption of new technologies or improved chemistries in a forensics laboratories. Any tools or tips that can reduce the barrier of validation, may also help the field of DNA forensics implement new technologies more quickly.

On October 1, Oskar Hansson, from the Department of Forensic Medical Services at Oslo University Hospital, will be leading a workshop entitled “Efficient Validation Using STR-Validator” as part of ISHI 28. This workshop introduces the free, open-source STR-Validator software tool that is designed to assist forensic laboratories in the evaluation of validation data. STR-validator is a free and open source R-package developed mainly for internal validation of forensic STR DNA typing kit. However, it is equally suited for validation of other methods and instruments, or for process control. The graphical user interface of the software enables easy analysis of data exported from software programs like GeneMapper® software, without any knowledge about R commands. The software also provides convenient functions to import, view, edit, and export data. After completed analysis, the results, plots, heat-maps, and data can be saved for easy access. Currently, analysis modules for stutter, balance, drop-out, concordance, mixtures, precision, pull-up, result types, and analytical threshold are available. STR-validator can greatly increase the speed of validation by reducing the time and effort needed for analysis of the validation data.

The workshop will include lectures and demonstrations to introduce STR-Validator as an efficient tool for the analysis of validation data in accordance with ENFSI recommendations and SWGDAM guidelines. This workshop is suitable for DNA analysts, technicians and QA/QC managers.

Have you registered for ISHI 28 in Seattle? Check it out. This year’s panel discussion will take up the topic of familial searching. Preregister for workshops. Read speaker bios.

Interested in more tips for smoother validation in your lab? This blog has several suggestions.

Promega Third Party Forensic-Grade Certification

Posted on by Promega

Promega has become the first major forensic manufacturer to achieve third party certification of the published ISO 18385 standard to minimize the risk of human DNA contamination in products used to collect, store and analyze biological material for forensic purposes.

On February 2, 2016, ISO 18385:2016 was published as the first international standard specific to the forensic manufacturing community. Since the standard was published, companies have begun to self-declare that they comply with the ISO standard. Some companies have gone a step further and reached out to Certification Bodies to provide an unbiased and independent assessment their compliance to ISO18385 through a third-party audit.

When consumers see an ‘ISO 18385 Forensic Grade’ labeled product, it should inspire confidence that the product was produced in accordance with a minimum set of criteria common to all manufacturers.

So what are you actually getting in a Forensic Grade labeled product? Continue reading “Promega Third Party Forensic-Grade Certification”

Promega Tech Tours 2017: The Power to Solve for the Forensics Community

Posted on by Promega

Governor John Bel Edwards of Louisiana made an appearance at the Promega Technology Tour in Baton Rouge. Pictures courtesy of Forensic Scientists at the Louisiana State Police.

2017 finds Promega on the road visiting cities all across the United States. This year we are presenting workshops from leaders in the forensics community on topics like maximizing success with challenging samples, improving laboratory efficiency and reducing backlogs, and new tools and technologies for the forensics laboratory. This highly popular workshop series is a great way to learn from your peers about new techniques and workflows and network with other forensics experts in your region.

There are several more tours left between now and the end of 2017. Find out if we are coming to a city near you and register today!

Catching a Child Abuser in Five Days

Posted on by Promega

ishi_27jpg

Next week, forensic analysts from all over the world will gather in Minneapolis for the 27th  International Symposium on Human Identification (ISHI). So today, we’d like to share one story from a forensics lab that highlights the importance of collaboration, knowledge-sharing and technology development–since that is what ISHI is all about. 

Crimes against children are especially heinous, and it is vitally important that the offenders are removed from the streets as soon as possible. In today’s blog, Sarah Chenoweth from the Anne Arundel County Crime Lab in Maryland describes a sexual assault case that was solved in just five days. Key to this speed were the collaboration between lab staff and state police, and the ability to quickly and reliably amplify DNA profiles from low-DNA samples. Thanks to the efforts of the investigators involved, parents in Maryland, and possibly nationwide, can sleep a little easier.

On Friday, February 5th, the Anne Arundel County Crime Lab was notified of a sex offense involving a 7-year-old victim. With our efficient DNA workflow, including use of the Fusion amplification kit with our 3500 Genetic Analyzer, we were able to identify the perpetrator in only five days. Continue reading “Catching a Child Abuser in Five Days”