ProteaseMAX Surfactant: Enhanced In-solution Digestion Applications

ProteaseMax 11228MA

The primary advantage of ProteaseMAX™ Surfactant is that it improves identification of proteins in gel by enhanced protein digestion, increased peptide extraction, and minimized post digestion peptide loss. However, ProteaseMAX™ Surfactant can also facilitate in-solution digestion protocols.

ProteaseMAX™ Surfactant offers two major benefits for digesting proteins in solution.

First, ProteasMAX™Surfactant efficiently solubilizes proteins. In addition, ProteaseMAX™ Surfactant does not interfere with Mass Spec analysis when used in the recommended amount. Mouse membrane proteins can be solubilized with ProteaseMAX™ Surfactant at room temperature in less than 1 hour.

The second advantage of ProteaseMAX™ Surfactant for in-solution protein digestion is an enhanced rate of proteolysis. ProteaseMAX™ Surfactant can partially or fully denature proteins in an SDS-like manner, giving the protease easier access to cleavage sites.

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The following are references:

  1. Rauniyar, N. et al. (2013)  Stable isotope labeling of mammals (SILAM) for in vivo quantitative proteomic analysis. Methods 61, 260–8
  2. Yim, Y.S.  et al. (2013)  Sli9trks control excitatory and inhibitory synapse formation witb LAR receptor protein tyrosine phosphatases. Proc. Natl. Acad. Sci. 110, 4057–62.
  3. Winter, D.  and Steen, H. (2011) Optimization of cell lysis and protein digestion protocols for the analysis of HeLa S3 cells by LC-MS/MSProteomics 11, 4726–30.
  4. McNamara, M. et al. (2012) Surface Proteome of Mycobacterium avium during the Early Stages of Macrophage Infection.  Infection and Immunity 80, 1868–80.
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Gary Kobs

Gary Kobs

Gary earned his B.S. in Bacteriology, UW-Madison in 1982. From 1982–1986 he served as Research Tech at UW-Madison. From 1986 to the present Gary has been with Promega Corporation serving in many capacities including as the very first editor of Promega Notes. He was also Manager Tech Services and Training, Product Manager Restriction/Modifying Enzymes, Product Manager Protein Analysis, and Sr. Product Manager for Protein Analysis products. Gary has retired from Promega Corporation.

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