COVID-19 Intranasal Vaccines: Right on the Nose?

Last updated April 28, 2023

covid-19 intranasal vaccines

COVID-19 is still a global pandemic. Around the world, as of 5:40pm CEST, 26 April 2023, there have been 764,474,387 cumulative confirmed cases of COVID-19, including 6,915,655 deaths, reported to the World Health Organization. As of 24 April 2023, a total of 13,325,228,015 vaccine doses have been administered. The adoption of vaccines worldwide continues to increase, yet periodic spikes and surges in infection rates continue to occur with new SARS-CoV-2 variants, such as that observed in Australia in Jan 2022. Vaccine booster doses provide effective protection against developing severe disease and hospitalization, but vaccine adoption and distribution face ongoing challenges in low- and middle-income (LMIC) countries (1). The development of intranasal vaccines could help alleviate some of the challenges in these areas. Therapeutic interventions for those already infected are in development, with one (Paxlovid) currently available under emergency use authorization (EUA) in the US.

Cumulative COVID-19 statistics by country: WHO COVID-19 Dashboard. Geneva: World Health Organization, 2020. Available online: https://covid19.who.int/ (last cited: April 28, 2023).

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What You Need, When You Need It: Monitoring and Responding to Ongoing Supply Chain Disruptions

Today’s blog is written by Brady Musson, Director of Global Logistics at Promega.

Our priority is ensuring that Promega customers receive everything they need, exactly when they need it. As the Director of Global Logistics at Promega, that is my primary focus, and the expertise of my entire team. I want to assure you that we’re doing everything we can so you can focus on keeping your work moving.

There are many factors that go into realizing these goals, and I would like to take a moment to address how Promega is managing some of the recent challenges. Our long-term, sustainable approach to business helps us weather uncertainty and instability. Our strong network of relationships allows us to explore creative solutions when challenges arise. Above all, our employees are willing to do what it takes to support the important work being done by life scientists around the world.

Promega is working hard to ensure that supply chain disruptions do not affect scientists' abilities to further their work using Promega technologies.
Kepler Center is the home of Promega customer service, kit packaging and shipping.
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GloMax® Instrument and NanoBiT® Technology Pave the Way for New Research Lab

Dr. Alsulami and the GloMax® Navigator  in his new research lab.

When setting up a new research lab, many researchers opt to outfit their new space with the technology and materials that got them through their academic studies. After graduating with his PhD from the University of Wisconsin-Madison, Dr. Tawfiq Alsulami will be traveling with a GloMax® Navigator Microplate Luminometer across the globe to continue his work as Assistant Professor in the Department of Food Science and Nutrition at King Saud University. Dr. Alsulami and his new lab at KSU will be using the NanoBiT® system to develop novel assays for the future of food testing.

Dr. Tawfiq Alsulami answered a few of our questions regarding his upcoming research at KSU and offered a few pieces of advice to new labs. Read the Q&A below:

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iGEM Team Explores Regenerative Spinal Cord Treatment with Promega Support

Today’s blog is written by KCL iGEM Team Leaders Alya Masoud Abdelhafid and Luke Bateman. Both in their third and final year at King’s College London, Alya is completing a BSc in Nutrition and Luke a BSc in Biochemistry.  

Every year, the International Genetically Engineered Machine competition (iGEM) offers high-school, undergraduate and post-graduate students the opportunity to conduct independent research using synthetic biology and genetic engineering to develop solutions to local and global problems.

More than 350 teams from around the world participate in iGEM, which culminates in a presentation at the global iGEM Giant Jamboree, attended by more than 6000 people every year. Here, novel research is presented amongst pioneers in synthetic biology, and outstanding projects are awarded prizes for their contribution to the greater scientific community. iGEM teams consistently produce ground-breaking solutions to modern challenges, many of which facilitate the development of multimillion-dollar companies and start-ups.

We are the King’s College London (KCL) iGEM team and are honored to be involved in the innovative and prestigious iGEM community.

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Monitoring Cellular Metabolism for NAFLD/NASH Liver Disease Research

In this blog, Dr. Jolanta Vidugiriene, Senior Research Scientist at Promega Corporation, discusses tools for studying metabolism in NAFLD/NASH research.

Dr. Jolanta Vidugiriene, Senior Research Scientist at Promega Corporation, discusses tools for studying metabolism in NAFLD/NASH research

What is nonalcoholic fatty liver disease (NAFLD)?

NAFLD is not a simple disease, it is an umbrella term for a range of liver conditions. The main defining characteristic of NAFLD is fat accumulation in the liver, called steatosis. In about 20% of people, steatosis is accompanied by inflammation, which is a more severe form of NAFLD called NASH (nonalcoholic steatohepatitis). NASH can progress to more advanced conditions like liver cirrhosis and liver failure. Most of the time, NAFLD is associated with underlying conditions—it is closely related to metabolic dysfunction, obesity, and type 2 diabetes. To better reflect the disease pathology, there has been a lot of discussion in the field recently to rename NAFLD to MAFLD, for metabolic associated fatty liver disease. Even though NAFLD has been studied for many years, the causes and progression of the disease are still not well understood. There are no FDA-approved diagnostic tools or treatments for it yet.

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Finding the Right Maxwell® RSC Kit for Your Nucleic Acid Extraction

This blog was written by guest writers Paraj Mandrekar (Technical Services Scientist 3) and Michelle Mandrekar, (Research Scientist 4).

Here are some designer’s notes comparing the Maxwell® RSC Blood DNA and the Maxwell® RSC simplyRNA kit chemistries for nucleic acid extraction.

The Maxwell RSC Blood DNA Kit and Maxwell RSC simplyRNA Blood Kit were both developed from the same non-silica-based purification chemistry and use the same underlying paramagnetic particle. This chemistry is characterized by an extreme binding capacity (the capacity of nucleic acid that can be bound on the particle), leading to both chemistries being capable of isolating large amounts of nucleic acid volumes and then eluting into relatively small volumes (50 µL). It is not unusual with either chemistry to have isolates that exceed 100 ng/µL. Although the chemistries have several similarities, there are some important distinctions between how the two chemistries were designed that influence which kit you choose for your nucleic acid extraction.

Image of blood with molecules of DNA and RNA superimposed Nucleic Acid Extraction
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Finding the Latest Science News While On the Go

Today’s guest blog was written in collaboration with Melissa Martin, a former global marketing intern with Promega. She is a senior at the University of Wisconsin-Madison where she is double majoring in zoology and life sciences communication, with a certificate in environmental studies.

Have you ever found yourself wondering what the newest advancements were on genetically engineering plants or using artificial intelligence in biotechnology but didn’t know where to start looking? You most likely know the basic science behind the headlines, but a general web search may lead to dramatized articles that focus more on getting attention than being accurate. Or you might find a scholarly article that will offer in-depth, peer-reviewed information but may require more time to read than you are willing to give.

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Using Databases to Find Scholarly Sources

Today’s guest blog was written in collaboration with Melissa Martin, a former global marketing intern with Promega. She is a senior at the University of Wisconsin-Madison where she is double majoring in zoology and life sciences communication, with a certificate in environmental studies.

Peer-reviewed papers are considered the most technical and in-depth way to learn about research and scientific advances. As a student or scientist, you will not only want to read scholarly articles to learn about what others are doing in your field but also to expand your knowledge and learn about scientific advances in completely new areas of study. With countless disciplines of science covering wide-ranging topics such as cell biology, physical chemistry or human behavior, it can be overwhelming to do a general search and find articles and journals that will have the topics relevant to your interests.

Young woman searching databases for findingscholarly articles.
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Resume vs. CV — What Is the Difference?

Today’s guest blog is written by Sophie Mancha, a former global marketing intern with Promega. She is in her 4th year as a PhD candidate in the Biomedical Engineering Department at the University of Wisconsin-Madison, studying pancreatic cancer.

Whether you are applying for a scholarship or trying to land a position in a research lab, there are plenty of decisions to make. Arguably, the most critical consideration is what documents to include to showcase that you are the right person for the job. Specifically, should you be preparing a curriculum vitae (CV) or a resume? What exactly is the difference?

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How Can You Improve Protein Digests for Mass Spectrometry Analysis?

Can predigestion with trypisin (ribbon structure shown) improve protein digests for mass spectrometry analysis?
Can pre-digestion with trypsin improve mass spec analysis?

The trypsin protease cleaves proteins on the carboxyterminus of Arginine (Arg) and Lysine (Lys). This cleavage reaction leaves a positive charge on the C-terminus of the resulting peptide, which enhances mass spectrometry analysis (1,2). Because of this advantage, trypsin has become the most commonly used protease for mass spectrometry analysis. Other proteases which cleave differently from trypsin, yielding complementary data are also used in mass spec analysis: these include Asp-N and Glu-C , which cleave acidic residues, and chymotrypsin which cleaves at aromatic residues. The broad spectrum protease, proteinase K is also used for some proteomic analyses. In a recent study, Dau and colleagues investigated whether sequential digestion with trypsin followed by the complementary proteases could improve protein digests for mass spectrometry analysis.

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