Today’s post was written by guest blogger Anupama Gopalakrishnan, Global Product Manager for the Genetic Identity group at Promega.
Next-generation sequencing (NGS), or massively parallel sequencing (MPS), is a powerful tool for genomic research. This high-throughput technology is fast and accessible—you can acquire a robust data set from a single run. While NGS systems are widely used in evolutionary biology and genetics, there is a window of opportunity for adoption of this technology in the forensic sciences.
Currently, the gold standard is capillary electrophoresis (CE)-based technologies to analyze short tandem repeats (STR). These systems continue to evolve with increasing sensitivity, robustness and inhibitor tolerance by the introduction of probabilistic genotyping in data analysis—all with a combined goal of extracting maximum identity information from low quantity challenging samples. However, obtaining profiles from these samples and the interpretation of mixture samples continue to pose challenges.
MPS systems enable simultaneous analysis of forensically relevant genetic markers to improve efficiency, capacity and resolution—with the ability to generate results on nearly 10-fold more genetic loci than the current technology. What samples would truly benefit from MPS? Mixture samples, undoubtedly. The benefit of MPS is also exemplified in cases where the samples are highly degraded or the only samples available are teeth, bones and hairs without a follicle. By adding a sequencing component to the allele length component of CE technology, MPS resolves the current greatest challenges in forensic DNA analysis—namely identifying allele sharing between contributors and PCR artifacts, such as stutter. Additionally, single nucleotide polymorphisms in flanking sequence of the repeat sequence can identify additional alleles contributing to discrimination power. For example, sequencing of Y chromosome loci can help distinguish between mixed male samples from the same paternal lineage and therefore, provide valuable information in decoding mixtures that contain more than one male contributor. Also, since MPS technology is not limited by real-estate, all primers in a MPS system can target small loci maximizing the probability of obtaining a usable profile from degraded DNA typical of challenging samples. Continue reading “Is MPS right for your forensics lab?”
Today’s blog is contributed by guest blogger Caitlin Cavanaugh, Client Support Consultant with Promega North America.
Recently, I began a new role as a client support consultant at Promega. In this role, I’m responsible for all technical and sales support for the Promega portfolio in the New Jersey and Philidelphia area.
Before coming to Promega, I worked in a lab at a start-up company right out of college, then made my way into sales, where I worked for a leading life-science instrumentation company for thirteen years.
Held May 2018, Means and Metrics for Detecting and Measuring Consciousness was designed to explore emerging technologies for studying the phenomenon of consciousness, including research related to sleep, wakefulness, altered states, focused attention and coma. We asked the question: How might our ability to better measure consciousness create opportunities to improve human function, resolve disease states and keep the mind and brain throughput all stages of life?
In the United States, the last Monday of May is Memorial Day, a national holiday in which we honor those who have given their lives in service to the country. For those of us living in Wisconsin, Memorial Day is also usually preceded by the first truly warm weekend of summer. So as families remember their loved ones, they gather together to create new memories in parks and backyards, around picnic tables or on grassy lawns–beginning the summer season of cookouts, picnics and bar-b-ques.
Here at Promega we love a good cookout too. So a few of us have cobbled together some of our favorite summer recipes to share with you. Do you have a favorite summer recipe? Share it in the comments below. (Please note metric conversions are approximate and have not been tested.) Continue reading “Kicking Off Summer with Some Cookout Favorites”
Today’s Promega Connections blog is written by guest blogger Joliene Lindholm, Promega Technical Services Scientist.
In Promega Technical Services, we are frequently asked questions about choosing among our Human Genomic DNA products. Promega offers DNA that can serve as sources of normal human gene sequences or positive controls where genotype is not critical, and controls for use in genotyping applications like STR analysis. For mouse researchers, we also offer Mouse Genomic DNA. Continue reading “Which DNA Do I Use? How to Choose Your Control and Other DNA Samples”
Today’s Promega Connections blog is written by guest blogger Tori Sheldon, North America Marketing and Events Coordinator.
It is crazy to think how quickly the months fly by. It feels like yesterday I was watching the ball drop as 2017 turned to 2018. Now it is almost March, when Wisconsin starts to emerge from the cold winter. March also happens to be National Optimism Month.
As I think about optimism, I am reminded of one of the core values that guide interpersonal relationships at Promega: “look for the good, with discernment”. The spirit of this value is to remember that deep down everyone is trying to come from a positive place and that even though we may not always agree with each other it is an opportunity for further discussion and collaboration.
Today’s blog post is written by guest blogger, Josh Agate, Manager, Global CRM.
Adventure is relative. Most people are looking for new adventures in life, and those can range from planning where to go on vacation to starting a new job. What each person looks for in an adventure and the level of thrill they seek is different. When I learned that Promega had awarded me a trip to a destination of my choice with my family for my job performance, I was excited to plan this new adventure with my wife and two daughters (ages 4 and 6). We decided on a trip to Belize.
The trip required two commercial flights, followed by a puddle jumper flight (with hand-written boarding passes), and a 30 minute boat ride before we arrived at our hotel on the island of Ambergris Caye. This island, off the northern coast of Belize, would provide the backdrop for our family’s greatest adventure to date. The trip to get to the island wasn’t tedious travel for them; it was a wild ride that included a plane that held 12 people, flying over crystal clear waters and a boat trip, where our hair flew wildly as we were sprayed with ocean mist. Continue reading “Adventure in Belize”
We invite you to travel with Bettina Bazzini-Lapin, Scientific Client Specialist, who was awarded a Promega Travel Award for sales performance and used her award to travel to Croatia and Italy. In this blog, she describes her travels.
Croatia is an Eastern European country that sits on the Adriatic Sea directly across from Italy. It has more than one thousand islands, and a third of the country is covered by forest. It is known for its beautiful Dalmatian coast line. One of the main sites for travelers to visit is the coastal city of Dubrovnik, known as the Pearl of the Adriatic. This is where my adventure began. Continue reading “Promega Travel Award Blog: An Excursion to Croatia”
This blog post is contributed by guest blogger Diana Clark, Benefits Manager, Promega Corporation
November is National Family Caregivers Month, first proclaimed by President Clinton in 1997, the proclamation has been renewed by every U.S. President since. When President Obama proclaimed this designation in 2012, he commented, “The unselfish devotion of family caregivers affirms the importance of respecting the dignity of life in all stages and underscores the importance of the family unit.”
Hearing these words, I felt even prouder to be a part of the Promega family. You see, we are already in the process of rolling out Caregiver Leave for 2018. Caregiver Leave will provide Promega employees with an additional two weeks of paid time off annually to care for a sick parent, spouse or child, or to welcome a new child into their family via birth, adoption or foster placement. Continue reading “Honoring Caregivers”