Dual-Luciferase or Dual-Glo Luciferase Assay System? Which one should I choose for my reporter assays?

Confused womanI’ve got a set of experiments planned that, if all goes well, will provide me with the answer I have been seeking for months. Plus, my supervisor is eagerly awaiting the results because she needs the data for a grant application, so I don’t want to mess it up. However, I am faced with a choice for my firefly and Renilla luciferase reporter assays: Do I use the Dual-Luciferase® Reporter Assay System or Dual-Glo® Luciferase Assay System? What’s the difference? How do I decide which to use? I’m so confused! Help!

Sound familiar? Not to worry! The choice is not difficult once you know how these assays work and how they differ.
Continue reading “Dual-Luciferase or Dual-Glo Luciferase Assay System? Which one should I choose for my reporter assays?”

A Little Bioluminescence for the International Year of Light

22159-2268-CR-WC-NanolucWebNavImage280x1402015 is the International Year of Light, and activities around the globe are planned to celebrate light in nature, the scientists who have helped us understand the nature of light and the engineers who have developed countless tools and technologies harnessing the power of light. At Promega, our favorite kind of light in nature is bioluminescence. So your Promega Connections bloggers thought we would share this incredible National Geographic video of ocean bioluminescence. In this video, starlight cameras capture the bioluminescence of the ocean, revealing an amazingly beautiful lightscape that is invisible to the unaided human eye. Enjoy!

Interested in Learning More? Check out these Bioluminescence-Related Blog Posts:

Angel’s Glow: Bioluminescence on the Battlefield

Bioluminescence from the Sky to the Ocean’s Depths

Tracking the Progression of the Plague Using Bioluminescence

To inject or not inject?

GloMax® Discover Multimode Reader with injectors.
GloMax® Discover Multimode Reader with injectors.

Luciferase assays are useful tools for studying a wide range of biological questions. They can be performed easily by adding a reagent that provides components necessary to generate a luminescent signal directly to cells or a cell lysate. However, once this reagent has been added, how long you wait to measure the signal becomes a key consideration in generating consistent data. Dependent on which luciferase assay you use, you may need a luminometer that can use injectors to deliver the assay reagents. The reason for this is simple, but can be confusing to new users.

Let’s start by discussing two types of luciferase assays: “flash” vs. “glow”. Continue reading “To inject or not inject?”