I’ve got a set of experiments planned that, if all goes well, will provide me with the answer I have been seeking for months. Plus, my supervisor is eagerly awaiting the results because she needs the data for a grant application, so I don’t want to mess it up. However, I am faced with a choice for my firefly and Renilla luciferase reporter assays: Do I use the Dual-Luciferase® Reporter Assay System or Dual-Glo® Luciferase Assay System? What’s the difference? How do I decide which to use? I’m so confused! Help!
2015 is the International Year of Light, and activities around the globe are planned to celebrate light in nature, the scientists who have helped us understand the nature of light and the engineers who have developed countless tools and technologies harnessing the power of light. At Promega, our favorite kind of light in nature is bioluminescence. So your Promega Connections bloggers thought we would share this incredible National Geographic video of ocean bioluminescence. In this video, starlight cameras capture the bioluminescence of the ocean, revealing an amazingly beautiful lightscape that is invisible to the unaided human eye. Enjoy!
Interested in Learning More? Check out these Bioluminescence-Related Blog Posts:
Luciferase assays are useful tools for studying a wide range of biological questions. They can be performed easily by adding a reagent that provides components necessary to generate a luminescent signal directly to cells or a cell lysate. However, once this reagent has been added, how long you wait to measure the signal becomes a key consideration in generating consistent data. Dependent on which luciferase assay you use, you may need a luminometer that can use injectors to deliver the assay reagents. The reason for this is simple, but can be confusing to new users.