fc effector

Bringing Cutting Edge Technologies to Academic Researchers Through the Academic Access Program

Posted on by Promega

This post was written by guest blogger Iain Ronald, Director Academic/Government Market Segment at Promega.

My back story is similar to most of you reading this blog, high school education, undergraduate degree then onto a postgraduate degree. However, over 25 years ago during my undergraduate study, I was fortunate enough to work in the lab of Professor Ray Waters studying DNA damage in S. cerevisiae as a model organism and at the time PCR was cutting-edge technology and the PCR license was in full effect. However, there was one company that was fighting the good fight to democratize PCR for the good of the scientific community, Promega.

I became enamored with Promega then, and the next steps in my career were taken with a view to working at this company who, for all intents and purposes, seemed to really care about the progression of science beyond self-aggrandizement.

Now that I am working at Promega in a position where I can bring benefit to our academic community, I have pondered what I can do to equal the disruptive attitude I observed in this company all those years ago when they were fighting the then “big tech” for the enablement of the scientific community. 

Reporter bioassays are one of hte many offerings of the academic access program.
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How Can a ADCC Reporter Bioassay Help You Save Time and Reduce Variability?

Posted on by Kari Kenefick

Fc receptor-mediated antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) by which antibodies target diseased cells for elimination. Traditional methods for measuring ADCC require primary donor peripheral blood mononuclear cells (PBMCs) or purified natural killer (NK) cells that express Fc receptors on the cell surface. However, primary cultures of PBMCs and NK cells introduce variability, high background, and can be tedious to prepare. Using a commercially available ADCC reporter bioassay can overcome many of the limitations of these primary cell assays.

Our ADCC and ADCP Reporter Bioassays are biologically relevant, MOA-based assays that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate Fcγ receptors. The ADCC Reporter Bioassays use an alternative readout from traditional primary cell-based assays: the FcγR and NFAT-mediated activation of luciferase activity in the effector cells. Primary cells are replaced with a Jurkat cell line stably expressing human FcγR variant and NFAT-induced luciferase.

The thaw-and-use cell format of the ADCC Reporter Bioassay saves time and labor of primary cell assays, while reducing variability. While a primary cell assay can take 1-2 weeks from culturing cells to results, ADCC reporter bioassay can be performed in 3–24 hours. The bioassays include all of the required reagents and are easily amenable to high-throughput workflows, enabling you to have precisely the right throughput for your workflow needs.

Check out the full Promega portfolio of Fc effector reporter bioassays to discover the best tool for your research and read more about how these assays