Travelogue Galapagos Part I: Realizing a Lifelong Dream

Posted on by Promega

In 2014, Promega created a special incentive to reward field science consultants who help the scientific community take advantage of our on-site stocking program. The winners had to meet ambitious criteria to receive 2 round-trip tickets to anywhere in the world, a week of paid vacation and spending money. Our four winners will share photos and stories about their journeys on the Promega Connections Blog.

Today’s travelogue is Part I of the adventures of Amy Parman, a regional sales manager, who used her award to travel to Ecuador and the Galapagos Islands.32178451-dec-12-image-1-600x400-web

Day 1: Seattle to Miami – Today we tied up a few last minute details, tested the satellite phones, checked that Grandma and Grandpa had all the info and resources they needed for anything that should arise while we were inaccessible (the manual we left was slightly thicker than War and Peace, and way less interesting), loaded up the car, put on the out-of-office alerts, kissed the kiddos good-bye and headed to SeaTac for a red-eye flight to Miami. We’re off!

Day 2: Miami to Guayaquil, Ecuador – Today (Was it day? After a red-eye, who knows?) we arrived in Miami in the very wee hours. We snagged a quick breakfast and a hotel right in the Miami airport which rents rooms for the day, which was our first experience with an hourly hotel—ahem! Our nine hour layover included lunch, mani/pedis and a much needed nap. We boarded our flight to Guayaquil and made full use of some particularly goofy-looking, newly-purchased neck pillows. My husband created a little excitement as we boarded the plane—he couldn’t find his passport as the flight attendant was readying the flight doors for departure. Our local seatmates and two flight attendants helped to find it quickly as it had slipped between the window seat and the floor. Phew! The flight was smooth as a wet seal and we arrived in Guayaquil to find the Hilton shuttle waiting for us. We checked into our room and grabbed some midnight sangria and flautas while we listened to an awesome Ecuadorian jazz band in the hotel bar. This trip is off to a fabulous start! Continue reading “Travelogue Galapagos Part I: Realizing a Lifelong Dream”

Deciding What to Share: Evaluating Content in a Self-Publishing World

Posted on by Michele Arduengo, PhD
Copyright: cuteimage / 123RF Stock Photo
Copyright: cuteimage / 123RF Stock Photo

A BuzzFeed News analysis of “news” stories during the final three months of the 2016 US presidential campaign revealed that on Facebook, the 20 top-performing fake-news stories from hoax sites and hyper partisan blogs generated 8,711,000 instances of engagement (shares, reactions, or comments) while the 20 top-performing stories from news web sites generated 7,367,000 instances of engagement (1). Basically fake news generated 1.5 million more responses than real news.

This is particularly concerning given that a Pew Research study from July 2016 indicated that 63% of Americans say that family and friends are an important way they get news—they get their news from their social networks (online or offline) rather than from vetted broadcast or print media (2), and 54% of people asked in this same study responded that they “sometimes” or “often” received news from social networking sites such as Facebook or Twitter.

I too must confess that quite often it’s a tweet or a Facebook post that alerts me to a news story or world event. Often it’s even a tweet or a post that leads me to the latest science news. I can’t remember the last time I deliberately watched the 6:00 news, though it was a staple in my house when I was growing up.

So what does all of this mean for science communication, science literacy and a basic understanding of what is really going on in the world?

Continue reading “Deciding What to Share: Evaluating Content in a Self-Publishing World”

The Role of the NanoLuc® Reporter in Investigating Ligand-Receptor Interactions

Posted on by Nicole Sandler

Luminescent reporter assays are powerful research tools for a variety of applications. Last March we presented a webinar on this topic, Understanding Luminescent Reporter Assay Design, which proved to enlighten many who registered. The webinar addressed the importance of careful experimental design when using a luminescent reporter such as Promega’s Firefly or NanoLuc® Luciferase.

Reporters provide a highly sensitive, quantifiable metric for cellular events such as gene expression, protein function and signal transduction. Luminescent reporters have become even more valuable for live, real-time measurement of various processes in living cells. This is backed by the fact that a growing number of scientific publications reference the use of the NanoLuc® Luciferase reporter and demonstrate its effectiveness as a reporter assay. Continue reading “The Role of the NanoLuc® Reporter in Investigating Ligand-Receptor Interactions”

Promega Spain Gets Moving Outdoors For A Cause

Posted on by Karen Burkhartzmeyer

A bike ride through the beautiful countryside, or even a routine walk to the corner store, is good for both body and soul. But sometimes even that’s not enough to get us moving in the fresh air. So our Promega Biotech Ibérica branch recently found a way to raise the stakes, and make outdoor activity even more valuable for its employees.

Promega Biotech Ibérica collected images of the beautiful Spanish countryside during their Kilometros Solidarios campaign.
Promega Biotech Ibérica collected images of the beautiful Spanish countryside during their Kilometros Solidarios campaign.

Last summer, the branch came up with the idea to donate one euro to the children’s oncology department of Hospital La Paz in Madrid for every kilometer its staff biked, walked or ran outside. The idea behind Kilometros Solidarios (Solidarity Kilometers) was simple but powerful: healthy activity leading directly to the health of others.

The plan worked. During the months of July, August and September, employees got up and outside and the branch raised €1884 (about $1993 USD) toward the purchase of a much-needed portable ultrasound machine for the hospital. (To point out the obvious, 1884 kilometers of outdoor activity were racked up by just 15 employees. That’s 4710 times around an Olympic track, or about the same distance as walking, biking or running from Promega Ibérica to Promega GmbH or from the Promega Madison headquarters straight south to the Gulf of Mexico.) Continue reading “Promega Spain Gets Moving Outdoors For A Cause”

The Art of Eating: Embrace the Nap

Posted on by Promega

Celebrating Thanksgiving Copyright: rawpixel / 123RF Stock Photo
Celebrating Thanksgiving Copyright: rawpixel / 123RF Stock Photo

In the United States, Thanksgiving Day originated as an opportunity to give thanks for blessing of the harvest and to toast to a plentiful harvest the next year. Fitting with its origins, the modern Thanksgiving holiday is centered on food. Although we are grateful for this day of eating, why does it have to make us so sleepy?

L-tryptophan, commonly known as just tryptophan, is an amino acid found in many of the foods typically found at a Thanksgiving feast. You’ve probably heard one of your relatives cite it as the reason they fell asleep during the football game. Tryptophan is essential for the normal growth in infants and to balance nitrogen levels in adults. It is mostly found in proteins like turkey, chicken, dairy products and brown rice. Once the amino acid is consumed, the body converts it to 5-hydroxy-tryptophan (5-HTP), which is then turned into serotonin. Serotonin is the biochemical messenger that is associated with naps.

But tryptophan can’t do it alone. Tryptophan is not an essential amino acid, and it has to compete to get to the brain. Most of the time it gets kicked out of the way by other essential amino acids also traveling to the brain. So, why does it zip up to the brain on Thanksgiving? Carbohydrates. Continue reading “The Art of Eating: Embrace the Nap”

Bioassay for Cannabinoid Receptor Agonists Designed with NanoBiT™ Techology

Posted on by Kyle Hooper

Cannabinoids. What are they? Sometimes, Wikipedia can give a nice definition:

Tetrahydrocannabinol (THC), a partial agonist of the CB1 and CB2 cannabinoid receptors. Wikipedia Commons
Tetrahydrocannabinol (THC), a partial agonist of the CB1 and CB2 cannabinoid receptors. Wikipedia Commons

A cannabinoid is one of a class of diverse chemical compounds that acts on cannabinoid receptors in cells that alter neurotransmitter release in the brain. Ligands for these receptor proteins include the endocannabinoids (produced naturally in the body by animals), the phytocannabinoids (found in Cannabis and some other plants), and synthetic cannabinoids (manufactured artificially).

Synthetic cannabinoids (SCs) were originally created for the scientific investigation of two cannabinoid receptors, CB1 and CB2, but have made their way to the streets as “safe” and “legal” alternatives to marijuana.

The problem is that these SCs engage the cannabinoid receptors more completely and with higher affinity than anything derived from marijuana. As a result, SCs can produce serious side effects that often require medical attention. In fact, you are 30 times more likely to seek emergency medical attention following the use of an SC than with natural cannabinoid sources like marijuana.

Continue reading “Bioassay for Cannabinoid Receptor Agonists Designed with NanoBiT™ Techology”

From Napkin Sketch to “Custom Kit”: CloneWeaver® Workflow Builder Gets Your Cloning Organized

Posted on by Michele Arduengo, PhD

20161018_150403Let’s face it, most lab techs and purchasing agents aren’t all that happy when you send them an Instagram picture of your latest lunchroom-napkin cloning strategy as your order form for your next big cloning experiment. So we have created the CloneWeaver® Workflow Builder. You can transfer your brilliance easily from that lunchroom napkin to an orderly email or print out of every vector, enzyme, purification kit, and transfection reagent your next big molecular cloning experiment requires. You can even save your one-of-a-kind “cloning kit” for future endeavors.

The CloneWeaver® tool will walk you through every step of the molecular cloning process from selecting a vector to finding a transfection reagent for mammalian cells. So if you are starting a new project, we are with you every step of the way. We will help you find restriction enzymes and even remind you about markers and biochemicals that you may want to have on hand for your experiment. Within the tool we have links to additional resources like our RE Tool and catalog pages if you need more help.

clone_weaverAlready have a favorite vector and a freezer full of restriction enzymes? No problem, skip those steps and move on to getting the perfectly sized nucleic acid markers or the particular polymerase your experiment requires.

Are you teaching a molecular genetics course? CloneWeaver® workflow builder is perfect for creating the list of laboratory reagents you are going to need for your students—and you will have this same list as a starting point for other lab experiments or classes later on because you can save the lists that you build. You can even pass them along to other professors.

So, if molecular cloning is in your future, let us help you get organized. Try the CloneWeaver® Workflow Builder.

To Seq, or Not to Seq

Posted on by Nicole Sandler

Seq—shorthand for “sequence”— has become a more recognizable term thanks to a novel and provocative genomics initiative called the BabySeq Project. The project, officially launched in May 2015, was designed to explore the impact of whole-exome sequencing (WES) on newborn infants and their families. A randomized, controlled trial to sequence healthy and sick infants and then provide sequencing information, it is the first of its kind. Those infants randomized to receive WES undergo genetic sequencing of all protein-coding genes and analysis of about 1,700 genes implicated in childhood health, along with 18 years of follow up genetic counseling.29813751-nov-2-blog-post-nicole-600x470-web

The project is directed by Robert C. Green, geneticist and physician at Brigham and Women’s Hospital, Harvard Medical School and the Broad Institute, and Alan H. Beggs of Boston Children’s Hospital and Harvard Medical School. Funding, totaling $25 million, comes from the National Institute of Child Health and Development and the National Human Genome Research Institute. Continue reading “To Seq, or Not to Seq”

A Very Happy and BATTY Halloween

Posted on by Michele Arduengo, PhD
Crowds gather on the Congress Ave Bridge in Austin, TX to see the bat colonly.
Crowds gather on the Congress Ave Bridge in Austin, TX to see the bat colony.

My colleagues in the scientific communications group at Promega are pretty sure that I have bats in my belfry. And, they may be right. After all I have written extensively and repeatedly about bats in North America and the threat that they are facing from White Nose Syndrome, the devastating disease caused by a cold-loving fungus (you can read my last post here). And, just last week I skipped an awesome party on Rainey Street in Austin, TX, to instead hang out by the Congress Ave bridge in hopes of seeing the urban bats fly.

But just in time for the annual Promega Connections Halloween blog, I stumbled across some good news for our distant mammalian cousins.

Continue reading “A Very Happy and BATTY Halloween”

Royal Lessons for Scientific Discovery

Posted on by Robert Deyes

rdeyes-2This Summer I had the chance to travel to Isle Royale (‘Royal Island’) in Lake Superior for what was a trip that would challenge me in more ways than one.  Our group, comprising 3 adults and 5 boys, drove up to Copper Harbor in the Michigan Upper Peninsula and then made the four hour ferry crossing to the Isle Royale National Park.   We spent the next 6 days hiking across the island, covering a total of 50 miles with backpacks and tents weighing us down every step of the way.

The sights were fantastic, the weather tremendous and the sheer thrill of being unreachable by text, email or phone thoroughly satisfying to say the least.  But, like any adventure of this nature, there was an element of fear that accompanied the trip.  What if anyone got hurt on the island?  How would we handle losing all our food if critters raided our camp during the night?  Might someone get seriously sick on the ferry crossing?  Had we brought enough food to keep us healthy while not overloading our back packs with unnecessary weight that would hinder our progress?  What if our portable water filters got clogged up? Continue reading “Royal Lessons for Scientific Discovery”