A Researcher’s work is never easy but it is even harder when relative data are to be interpreted. This is especially true for Real-Time PCR. It is one of the most accurate ways to evaluate gene expression. However, despite it being such a powerful technique, it still carries many pitfalls which can lead a scientist to the wrong conclusion. Often a new user does not have thorough sample/RNA preparation, equipment or knowledge. So what are the considerations and aspects that the researcher should pay attention to? Continue reading “How to Choose a Good Reference Gene?”
PCR, qPCR, and RT-qPCR
Top Ten Things You Can Do to Improve Your PCR Results
10. Modify reaction buffer composition to adjust pH and salt concentration.
Continue reading “Top Ten Things You Can Do to Improve Your PCR Results”