Studying Mitochondrial Fission with NanoBiT Complementation Assay

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Vote for NanoBiT™ in the Scientist’s Choice Awards and get a chance to win a $500.00 US Amazon voucher from Select Science.

Motivation
It’s a new year. Whether you’re a self-improvement fanatic or just ready for good things to start happening, you’ve got a plan. You might be changing up an old exercise routine or trying a new cooking technique.

And at work, you are digging deeper; this is the year you illuminate the protein interactions that you’ve previously not been able to visualize.

Good news. There is a new protein complementation assay that can help. Studying-mitochondrial-fission-poster

About NanoBiT
NanoBiT™ Complementation Reporter is a recently developed protein interaction assay that features the improved NanoLuc® luciferase. NanoLuc, originally isolated from a deep sea shrimp, is a small luciferase that provides a much brighter signal than firefly luciferase.

About Split Luciferase Systems
If you’re interrogating two proteins to understand the conditions under which they interact, a split luciferase system enables you to tag each protein with a luciferase subunit. Interaction of the tagged proteins facilitates the complementation of the subunits, resulting in a luminescent signal. Continue reading