You’ve probably been there. You’ve got a new antibody or you’re testing out one you’ve made yourself. After weeks or months of work, your antibody is going to help move your research project forward. As you excitedly head to the dark room to develop your film, your mood is crushed when you see…bands, more bands, and smears. Alas, science has played one more cruel joke on you as you experience what so many of your fellow scientists have before. Despite such a dismal beginning, you often can still get good western blots by changing steps in your protocol.
You and I know that bacterial resistance to antibiotics is a serious and growing problem. As the Center for Disease Control notes, the problem is not only one of industrial nations, but is found worldwide:
For instance, we hear frequently of cases of methicillin-resistant Staphylococcus aureus or MRSA. Methicillin was widely regarded as an antibiotic of last resort in fighting serious S. aureus infections and, well, S. aureus has now done an end-run around methicillin.