Fc receptor-mediated antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) by which antibodies target diseased cells for elimination. Traditional methods for measuring ADCC require primary donor peripheral blood mononuclear cells (PBMCs) or purified natural killer (NK) cells that express Fc receptors on the cell surface. However, primary cultures of PBMCs and NK cells introduce variability, high background, and can be tedious to prepare. Using a commercially available ADCC reporter bioassay can overcome many of the limitations of these primary cell assays.

Our ADCC and ADCP Reporter Bioassays are biologically relevant, MOA-based assays that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate Fcγ receptors. The ADCC Reporter Bioassays use an alternative readout from traditional primary cell-based assays: the FcγR and NFAT-mediated activation of luciferase activity in the effector cells. Primary cells are replaced with a Jurkat cell line stably expressing human FcγR variant and NFAT-induced luciferase.

The thaw-and-use cell format of the ADCC Reporter Bioassay saves time and labor of primary cell assays, while reducing variability. While a primary cell assay can take 1-2 weeks from culturing cells to results, ADCC reporter bioassay can be performed in 3–24 hours. The bioassays include all of the required reagents and are easily amenable to high-throughput workflows, enabling you to have precisely the right throughput for your workflow needs.

Check out the full Promega portfolio of Fc effector reporter bioassays to discover the best tool for your research and read more about how these assays