Ah, the wonders and frustrations of cloning. We’ve all been there. After careful planning, you have created the cloned plasmid containing your DNA sequence of interest, transformed it into bacterial cells and carefully spread those cells on a plate to grow. Now you stand at your bench gazing down at your master piece: a plate full of tiny bacterial colonies. Somewhere inside those cells is your DNA sequence, happily replicating with its plasmid host. But wait – logic tells you that not ALL of those colonies can contain your plasmid. There must be hundreds of colonies. Which ones have your plasmid? You begin to panic. Visions of yourself old and grey and still screening colonies flash through your mind. At the next bench, your lab-mate is cheerfully selecting colonies to screen. Although there are hundreds of colonies on her plate as well, some are white and some are blue. She is only picking the white colonies. What does she know that you don’t? Continue reading “Selecting the Right Colony: The Answer is There in Blue and White”
In my science blog research/writing, news reports are usually pulled from US sources. But interesting scientific research is obviously being conducted in many places around the globe. When this story from Namibia came along, there was so much I didn’t know. It was time to catch up.
Namibia is Exactly Where in Africa?
Namibia is one of the world’s youngest countries, having gained independence from South Africa in 1990. Situated northwest of the country of South Africa on the Atlantic Ocean, Namibia is arid, composed largely of desert.
This blog is about research conducted at the Sam Nujoma Research Center, University of Namibia, on Henties Bay. Henties Bay (not shown on this map) is in the region of Erongo, located in the center of Namibia along the coast. Henties Bay has become a tourist destination in part due to the abundance of fish and marine life found there.
Today’s blog is brought to us by and alumus of Dane County Youth Apprenticeship Program, Aidan Holmes.
In this blog I have the opportunity to write about how my experiences at the BTC Institute as a high school student were instrumental in leading me to my passion for science education, my Peace Corps experience, and my current role as a biotechnology instructor for the very same institute.
I became familiar with the BTC Institute as a student at Marshall High School when our biology teacher organized a biotechnology field trip for us. I loved learning about DNA and biotechnology since 7th grade so attending a field trip like this was an incredible opportunity to engage in hands-on biotechnology. When I learned about the Youth Apprenticeship Program in Biotechnology I knew I had to apply and enrolled during my senior year of high school. Through the program I took a weekly class at the BTC Institute and I worked as a student researcher in a biochemistry lab at UW-Madison. I enrolled for classes at UW-Madison the following year and pursued an undergraduate degree in genetics and a certificate in education and educational services. Continue reading “From BTCI to Africa and Back Again: One Student’s Journey in Science Education”
The 2019 iGEM Competition is on the horizon and team registration opens this month. We’re excited to partner with the iGEM Foundation again this year and offer our support to the young scientists who participate. If you’re starting an iGEM project, there are going to be things you need along the way. We are pleased to share a number of different ways we can help your iGEM team from now until the Giant Jamboree.
Tell us about your iGEM project and your team could win a 2019 Promega iGEM Grant Sponsorship. Ten winning teams will each receive $2000 in free Promega products to use for their iGEM projects. Tell us about your project—What problem are you addressing? What is your proposed solution? What challenges does your team face? Last year’s winning teams selected from a wide range of reagents and supplies, including master mix, restriction enzymes, ligase, DNA purification kits, expression systems, DNA ladders and markers, buffers and agarose. Click here to apply! Continue reading “It’s Almost iGEM Season—Help Is On The Way!”
If you’re active on #sciencetwitter, you may have seen a thread recently about tautonyms. “Tautonym” is a cool word for scientific names where the genus and species are the same word, For example, Vulpes vulpes is the scientific name for the red fox.
I have taken great delight in sharing these tautonyms with friends, colleagues, and random strangers on the bus. However, the problem that I keep having is that people want more details about something than the name. If you’ve had that problem, too, then this blog is for you. Continue reading “Extra extra: Read All About Tautonyms”
Embryonic stem cells have the extraordinary ability to divide without limit yet maintain the potential to make all types of cells found in the human body. This holds tremendous implications for the worlds of drug discovery and testing, cell production, and tissue transplantation medicine.
Overall, I’m really glad I decided to go to the talk. I got to learn a lot about stem cells, how they are used in different parts of the body, and some of the difficulties with using stem cells. It was definitely way more enjoyable than anything else I was planning on doing during that time. If there are more opportunities like this that come up, I would definitely try to go to them.
Celebrating the 20th anniversary of Dr. James Thomson’s breakthrough work with induced pluripotent stem cells, the Wisconsin Institute for Discovery (WID) hosted a panel of University of Wisconsin stem cell scientists to discuss the future of their research on November 13th. Entitled “Stem Cell Science: The Next 20 Years” and designed for the general public, the audience heard from Drs. Lynn Allen Hoffman, David Gamm and David Vereide, who talked about applying stem cell research to develop clinical applications for skin grafting, vision restoration and regenerative biology, respectively.Continue reading “Dane County High School Students Attend Stem Cell Conference at Wisconsin Institutes for Discovery”
We have published 130 blogs here at Promega this year (not including this one). I diligently reviewed every single one and compiled a list of the best 8.5%, then asked my coworkers to vote on the top 5 out of that subset. Here are their picks:
No surprises here, everyone loves water bears. Kelly Grooms knows what the people want.
As adults, we can all attest to the benefits of attending professional conferences. They provide us with opportunities to present and share with others, network, and renew and refresh in our field. For some of us, that first conference, at the college or early employment level, may have contributed significantly to a sense of ourselves as professionals. But what does it mean to someone younger?
Recently, three high school students enrolled in the Dane County Biotechnology Youth Apprentice (YA) Program were able to conferences related to their interest in pursuing Science, Technology, Engineering and Math (STEM) careers. Here’s what they discovered. Continue reading “Conferences Are Important for High School Students—Youth Apprentices and STEM Professional Development”
This past weekend, I had the opportunity to be a part of “Once Upon a Christmas Cheery in the Lab of Shakhashiri”. Bassam Z. Shakhashiri is a professor of chemistry at the University of Wisconsin–Madison who is well-known for his fun science demonstrations and a fervent dedication to public science communication. Once Upon a Christmas Cheery started in 1970 as an end-of-semester treat for Dr. Shakhashiri’s freshman chemistry class; by 1973, the Christmas lecture had become so popular that Wisconsin Public Television offered to broadcast it during Christmas week, and this collaboration has continued uninterrupted ever since.
That’s 49 years of Christmas lectures, commemorated by making indium, the 49th element, the Sesame Street-esque “sponsor” of the show. It helps that indium burns bright violet, the name of Dr. Shakhashiri’s granddaughter and hence his favorite color. The color purple made a firm foundation for many aspects of the show: The chrysanthemums frozen in liquid nitrogen were purple, as was the balloon I inflated during my spiel on air movement. Most of the set was various shades of purple, too.
The South Pole was exactly as I expected—snowy and barren, apart from the giant research station in front of me. Suddenly, I got a notification in my communication system that there was a strong signal coming from the sky. I looked up and changed the visual display settings of my goggles to find stunning views of the Solar System, all the way past Pluto. My heads-up display told me that I’ve discovered a subatomic particle, called a neutrino, that flies through the fabric of space at nearly the speed of light. I wanted to find the source of this neutrino, so I switched my display to X-ray vision. The signal brightened, and the source was revealed—a massive black hole. I captured as much data as possible so I could report back to the lead scientist on the project. What an exciting afternoon of research!
Okay, I’ve never actually been to the South Pole, but I experienced this event in virtual reality at a conference expo booth for the National Science Foundation. This experience put me in the shoes of an astrophysicist working at the IceCube Neutrino Detection Facility, operated by UW-Madison researchers. As someone who specializes in the life sciences, I had the opportunity to learn more about an area outside my expertise—the fascinating world of particle physics.
Most people think of augmented reality (AR) and virtual reality (VR) in the context of gaming or entertainment. You’ve likely had a casual AR experience if you’ve ever given yourself a flower crown in Snapchat, or hunted for Charmander at your local park with the Pokémon GO app. Yet, as I experienced at a conference several weeks ago, AR and VR can have massive implications for education and training experiences in the sciences. Continue reading “Virtual Reality Is Changing How We Experience Science”