You are studying the effects of a compound(s) on your cells. You want to know how the compound affects cell health over a period of hours, or even days. Real-time assays allow you to monitor cell viability, cytotoxicity and apoptosis continuously, to detect changes over time.
Why use a real-time assay? A real-time assay enables you to repeatedly measure specific events or conditions over time from the same sample or plate well. Repeated measurement is possible because the cells are not harmed by real-time assay reagents. Real-time assays allow you to collect data without lysing the cells.
Advantages of Real-Time Measurement Real-time assays allow you to:
The ability to analyze more than one cellular biomarker in a single sample is advantageous for a number of reasons. Multiplexing allows researchers to save money and time, while conserving precious samples. In addition, understanding the relationship between cell biomarkers can provide a more complete picture of cell health that can lead to improved predictive models for drug discovery. Understanding biomarker relationships can also minimize ambiguity in the data set and validate if a treatment effect is real or an artifact of the system. To avoid repeat experiments and extract the most biologically relevant data from multiplex assays, consider these tips when performing multiplex cell-based assays. Continue reading “Tips for Multiplex Cell-Based Assay Success”
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