Q: Can PCR products generated with GoTaq DNA Polymerase be used to for T- vector cloning?
A: Yes. GoTaq® DNA Polymerase is a robust formulation of unmodified Taq Polymerase. GoTaq® DNA Polymerase lacks 3’ →5’ exonuclease activity and displays terminal transferase activity that adds a 3′ deoxyadenosine (dA) to product ends. As a result, PCR products amplified using GoTaq® DNA Polymerases (including the GoTaq® Flexi and GoTaq® G2 polymerases) will contain A-overhangs which makes them suitable for T-vector cloning with the pGEM®-T (Cat.# A3600), and pGEM®-T Easy (Cat.# A1360) Vectors.
Continue reading “T-Vector Cloning: Questions, Answers and Tips”
Do you count colonies on agar plates? Do you often need to average counts over a series of plates? The Promega Colony Counter app for iPhone® (3GS, 4S, and 5) and iPod® Touch (4th and 5th generation) allows you to take a picture of your plate, obtain a good first-guess count and refine it quickly by marking additional colonies and masking areas where the app may have over-counted.