T-Vector Cloning: Questions, Answers and Tips

Blue/White colony screening helps you pick only the colonies that have your insert.

Q: Can PCR products generated with GoTaq DNA Polymerase be used to for T- vector cloning?

A: Yes. GoTaq® DNA Polymerase is a robust formulation of unmodified Taq Polymerase. GoTaq® DNA Polymerase lacks 3’ →5’ exonuclease activity and displays terminal transferase activity that adds a 3′ deoxyadenosine (dA) to product ends. As a result, PCR products amplified using GoTaq® DNA Polymerases (including the GoTaq® Flexi and GoTaq® G2 polymerases) will contain A-overhangs which makes them suitable for T-vector cloning with the pGEM®-T (Cat.# A3600), pGEM®-T Easy (Cat.# A1360) and pTARGET™ (Cat.# A1410) Vectors.

Continue reading “T-Vector Cloning: Questions, Answers and Tips”

The 5 Stages of Failed Cloning Grief (and how to get back on track!)

Cloning is a fickle process that can make even the most seasoned bench scientists scream in frustration. By the time you perform a colony PCR and run the gel to check for your insert, you’ve invested several days in preparing these transformed cells. But then, the unthinkable happens. When you image your gel…the target band is missing.

This can trigger what’s known as “The 5 Stages of Failed Cloning Grief.” As you work through each stage at your own pace, just know that scientists all over the world feel your pain and can empathize with you in this difficult time. Continue reading “The 5 Stages of Failed Cloning Grief (and how to get back on track!)”

Now Available for Purchase: Promega Colony Counter App

colony counterDo you count colonies on agar plates? Do you often need to average counts over a series of plates? The Promega Colony Counter app for iPhone® (3GS, 4S, and 5) and iPod® Touch (4th and 5th generation) allows you to take a picture of your plate, obtain a good first-guess count and refine it quickly by marking additional colonies and masking areas where the app may have over-counted.

The app is available for purchase for 3.99 USD from the iTunes store in North America and Europe.