What happens when you engineer a small, super bright luciferase? A generation of new tools. We’ve highlighted many of the papers and new applications that NanoLuc® luciferase has enabled on this blog. Introduced first as a reporter enzyme to assess promoter activity, NanoLuc® has since become the foundation for bioluminescent tools that reveal endogenous protein dynamics, target engagement, protein degradation, immunodetection and more. Much of that biology was largely invisible before these tools existed. So where did NanoLuc® come from, and how does one enzyme power so many research capabilities? Read on for a primer on the technologies and applications built from this enzyme over the last decade.
Continue reading “NanoLuc® Luciferase Powers More than Reporter Assays”
