Top 10 Things that Might Improve Your qPCR or RT-qPCR Assays

headacheAlmost from the moment the science recognized the value of PCR amplification, it has been a bit of a love-hate relationship. One of the latest additions to the PCR portfolio, real-time or quantitative PCR (qPCR), can be an amazingly powerful tool. However, just like traditional PCR, qPCR can be frustrating. There are a number of parameters that can influence the success of your qPCR assay. Below I have highlighted ten things to consider when trying to improve your qPCR results. Continue reading