![3D depiction of NanoBiT Protein Complementation](https://www.promegaconnections.com/wp-content/uploads/2017/01/21416852-DC-CR-NanoBiT-Technical-Imagery.jpg)
Updated February 15, 2021
If you’re interrogating two proteins to understand the conditions under which they interact, a complementation system enables you to tag each protein. Interaction of the tagged proteins facilitates the complementation of the subunits, resulting in a signal. Here we discuss the NanoBiT complementation assay and describe its use to study mitochondrial fission.
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