Glowing Testimonies: A Review of NanoLuc® Use in Model Organisms


Model organisms are essential tools in the pursuit of understanding biological processes, elucidating the mechanisms of diseases, and developing potential treatments and therapies. Use of these organisms in scientific research has paved the way for groundbreaking discoveries across various fields of biology. In particular, non-mammalian models can be valuable due to characteristics such as rapid life cycles, low cost, and amenability to use with advanced genetic tools, including bioluminescent reporters such as NanoLuc® Luciferase.

NanoLuc® is a small (19.1 kDa) luciferase enzyme originating from deep sea shrimp that is 100x brighter than firefly or Renilla luciferase. It utilizes a furimazine substrate to produce its bright glow-type luminescence. In the decade following its development, the NanoLuc® toolbox has expanded to include NanoBiT® complementation, NanoBRET™ energy transfer methods, and new reagents such as the Nano-Glo® Fluorofurimazine In Vivo Substrate (FFz) which was designed for in vivo detection of NanoLuc® Luciferase, NanoLuc® fusion proteins or reconstituted NanoBiT® Luciferase. In addition to the aqueous-soluble reagents increased substrate bioavailability in vivo, with fluorofurimazine, NanoLuc® and firefly luciferase can be used together in dual-luciferase molecular imaging studies.

Here we spotlight some recent research that demonstrates how the expanded NanoLuc® toolbox can be adapted to use in non-mammalian models, shedding new light on fundamental biological processes and advancing our understanding of complex mechanisms in these diverse organisms.

Continue reading “Glowing Testimonies: A Review of NanoLuc® Use in Model Organisms”

Worms from Heaven and Hell

C. elegans were recently part of an experiment on the International Space Station.
Worms from the heights of space and the depths of the earth were in the news last week, one well-known species soaring to the heavens as part of a space flight experiment and a previously undiscovered species revealing the surprising extent of multicellular life in the hidden depths of earth.

The Worm from the Heavens
Caenorhabditis elegans perhaps qualifies as the most well-known of all worms. This 1mm roundworm, is a staple model organism in molecular biology. It’s easy to grow and store, possesses a simple neuronal network, and is transparent, making it easy to study cell differentiation and development. It was the first multicellular organism to have its genome sequenced, and the developmental fate of all its somatic cells has been studied. In some ways we know C. elegans better than we know ourselves.
Continue reading “Worms from Heaven and Hell”