What Shelter Dogs Can Tell Us About Emerging Zoonotic Diseases

Why Are Zoonotic Diseases Becoming a Bigger Risk?

As of September 9, 2025, the Worldometer listed the human global population as 8.3 billion people (1). This population growth means that humans will be living and working in previously uninhabited or minimally disturbed environments, increasing interactions between humans, domestic animals, wildlife, and their pathogens. This intensifying human-animal interface heightens the risk of zoonotic disease transmission, where pathogens cross species barriers (from wildlife to domestic livestock or from wildlife to humans), potentially leading to outbreaks and even pandemics.

How Do Urbanization and Climate Change Amplify Zoonotic Threats?

Urbanization, habitat disruption, and climate change further exacerbate these risks by altering ecosystems and facilitating the spread and emergence of vector-borne and zoonotic diseases. Understanding and addressing these threats requires robust surveillance, effective diagnostics, and proactive strategies to prevent and mitigate disease emergence and spread.

In urban areas, public health officials are already using wastewater to monitor known pathogens and identify “hot spots” of activity to predict increases in illness within local populations (2). Animal shelters are another place where there is an opportunity to monitor for emerging infectious diseases that could affect domestic pet animals.

Continue reading “What Shelter Dogs Can Tell Us About Emerging Zoonotic Diseases”

Developing an Experimental Model System to Understand the Tumor Microenvironment of Melanoma Brain Metastases

Cancer’s greatest threat is its ability to spread to other tissues—a process known as metastasis. Melanoma, a form of skin cancer, exemplifies this devastating progression. Although treatable when caught early—with surgical removal resulting in over 99% survival at five years—once melanoma metastasizes, five-year survival rates plummet dramatically to around 27%. Even more concerning, melanoma exhibits a particularly high tendency to invade the central nervous system, causing melanoma brain metastases (MBMs) that are incurable and reduce median survival to just 13 months.

To understand metastasis, we need reliable and realistic experimental models. Traditional cell cultures on plastic dishes are limited, failing to replicate the intricate spatial organization and biochemical interactions within living tissues. Animal models are informative but expensive, ethically complex, and not always accurate for human diseases. Addressing this critical gap, Reed-McBain and colleagues (2025) introduced an innovative microphysiological system (MPS) designed to simulate the tumor microenvironment in the brain affected by metastatic melanoma.

Continue reading “Developing an Experimental Model System to Understand the Tumor Microenvironment of Melanoma Brain Metastases”

IL-6/STAT3-Regulated Long Non-Coding RNA Is Involved in Colorectal Cancer Progression

Researchers from Wenzhou Medical University in China have identified a mechanism involving long non-coding RNAs (lncRNA) that contributes to colorectal cancer (CRC) progression. CRC is the third most common cancer worldwide and is one of the most lethal cancers across the globe. Understanding the molecular mechanisms that underlie the development and progression of CRC is critical to developing biomarkers to detect it and new therapeutics to treat it. 

Continue reading “IL-6/STAT3-Regulated Long Non-Coding RNA Is Involved in Colorectal Cancer Progression”

Using Dual-Luciferase Assays to Identify the Role of Non-Coding RNAs in Disease

In recent years, non-coding RNAs—especially microRNAs (miRNAs) and long non-coding RNAs (lncRNAs)—have emerged as powerful regulators of cellular behavior. These molecules modulate gene expression, often by targeting mRNAs for translational suppression or degradation. Two recent studies—one focused on osteoarthritis and the other on 5-Fu-resistant colorectal cancer—illustrate how these non-coding, regulatory RNAs operate within disease-relevant signaling networks, providing new points for therapeutic intervention.

lncRNA, long noncoding RNA

Both studies use the pmirGLO Dual-Luciferase miRNA Target Expression Vector to evaluation predicted miRNA activity.  This dual-luciferase system offers a clean and quantifiable way to validate miRNA–mRNA interactions using a simple bioluminescent readout. By cloning the 3´ untranslated regions (UTRs) of suspected targets downstream of a firefly luciferase reporter and normalizing against Renilla luciferase, researchers can rapidly confirm whether a miRNA directly regulates its target.

Continue reading “Using Dual-Luciferase Assays to Identify the Role of Non-Coding RNAs in Disease”

An Unexpected Role for RNA Methylation in Mitosis Leads to New Understanding of Neurodevelopmental Disorders

Traditionally, RNA methylation has been studied in the context of gene expression regulation, RNA stability and translation efficiency, with its primary role thought to be in modulating cellular homeostasis and protein synthesis. However, a 2025 study by Dharmadkikari and colleagues uncovers an unexpected and critical function for RNA methylation in mitotic spindle integrity.

False color transmission electron microscope (TEM) micrograph of a mitotic cell in metaphase stage showing chromosomes (purple) in the equatorial plane and one of the mitotic spindle poles (blue). Mutations in SPOUT1/CENP-32 affect RNA methylation which is necessary for proper cell division.
False color transmission electron microscope (TEM) micrograph of a mitotic cell in metaphase stage showing chromosomes (purple) in the equatorial plane and one of the mitotic spindle poles (blue).

The study identifies a critical role for SPOUT1/CENP-32-dependent methylation in mitotic spindle formation and accurate chromosome segregation. Originally identified in a large-scale analysis of proteins associated with mitotic chromosomes, SPOUT1/CENP-32 encodes a putative RNA methyltransferase. The protein localizes to mitotic spindles, and when it is absent centrosome detachment from the spindle poles, delayed anaphase, and chromosome segregation errors are observed. Further, CRISPR experiments in human cells show that the protein is essential for cell viability.

Continue reading “An Unexpected Role for RNA Methylation in Mitosis Leads to New Understanding of Neurodevelopmental Disorders”

Unlocking the Secrets of ADP-Ribosylation with Arg-C Ultra Protease, a Key Enzyme for Studying Ester-Linked Protein Modifications 

Post-translational modifications of proteins are critical for proper protein function. Modifications such as phosphorylation/dephosphorylation can act as switches that activate or inactivate proteins in signaling cascades. The addition of specific sugars to membrane proteins on cells are critical for recognition, interaction with the extracellular matrix and other activities. While we know volumes about some types of protein modifications, ADP-ribosylation on aspartate and glutamate residues has been more difficult to study because of the chemical instability of these ester-linked modifications. 

Matić Lab (Eduardo José Longarini and Ivan Matić) recently published a study that explored mono-ADP-ribosylation (ADPr) on aspartate and glutamate residues by the protein PARP1 and its potential reversal by PARG. PARP1 and PARG signaling are central to DNA repair and apoptosis pathways, making them potentially powerful therapeutic targets in cancer or neurodegenerative diseases in which DNA repair processes are often disrupted. 

Continue reading “Unlocking the Secrets of ADP-Ribosylation with Arg-C Ultra Protease, a Key Enzyme for Studying Ester-Linked Protein Modifications “

Exploring the Respiratory Virus Landscape: Pre-Pandemic Data and Pandemic Preparedness

influenza viruses are part of the worldwide respiratory virus landscape

Since the COVID-19 pandemic, public health researchers and research scientists have sought more urgently to understand the worldwide respiratory virus landscape. The COVID-19 pandemic has forced us to re-evaluate our global public health priorities and activities. Additionally, acute respiratory tract infections are one of the leading causes of illness and death worldwide, particularly in developing countries. To really understand what changed with the pandemic and how we can best respond going forward, we need to understand what the baseline landscape was before the pandemic. Studies using samples that were collected prior to the pandemic are essential to this effort.

Continue reading “Exploring the Respiratory Virus Landscape: Pre-Pandemic Data and Pandemic Preparedness”

From Fins to Genes: DNA Barcoding Unlocks Marine Diversity Along Mozambique’s Coast

DNA Barcding unlocks marine diversity along Mozambique's coast

The Mozambique Channel, which is located between the Madagascar and Mozambique on the African coast, is an important hot spot for biodiversity because its many coastal ecosystems provide a range of habitats that support diverse plant and animal species. Understanding the biodiversity of an ecosystem, particularly biodiversity hot spots, is important for many reasons. For marine systems, accurate classification and reporting of fish species supports fisheries research, natural resource surveys, forensic studies, conservation studies, and enables discovery of new or under-reported species. Studies have been limited along the west coast of Africa and are only now in their early stages.

A 2024 research study by Muhala and colleagues applied DNA barcoding to evaluate the composition of marine and coastal fish diversity from the Mozambican coast. In the study, the Wizard® Genomic DNA Purification Kit was used to extract DNA from both teleost (ray-finned) and elasmobranch (sharks, rays and skates) fish classes, with a total of 143 species sampled from local artisanal fisheries along the Mozambican coast. The samples were primarily composed of muscle or fin tissues, which are ideal for genetic analysis due to their higher DNA yield. These tissue samples were collected from various fish species captured along the coast of Mozambique, stored in ethanol (96%) to preserve DNA integrity, and then processed using the Wizard kit. Total genomic DNA was extracted from the muscle or fin tissues, as per the manufacturer’s protocol. This method ensures the isolation of high-quality genomic DNA, which is crucial for subsequent polymerase chain reaction (PCR) amplification and sequencing. The COI gene (cytochrome c oxidase subunit I) was targeted for DNA barcoding, enabling species identification and assessment of genetic diversity.

Continue reading “From Fins to Genes: DNA Barcoding Unlocks Marine Diversity Along Mozambique’s Coast”

The Buzz on Biodiversity: Exploring Pollinator Diversity Through Mitochondrial DNA Analysis

Almost three-quarters of the major crop plants across the globe depend on some kind of pollinator activity, and over one-third of the worldwide crop production is affected by bees, birds, bats, and other pollinators such as beetles, moths and butterflies (1). The economic impact of pollinators is tremendous: Between $235–577 billion dollars of global annual food production relies on the activity of pollinators (2).  Nearly 200,000 species of animals act as pollinators, including some 20,000 species of bees (1). Some of the relationships between pollinators and their target plants are highly specific, like that between fig plants and the wasps that pollinate them. Female fig wasps pollinate the flowers of fig plants while laying their eggs in the flower. The hatched wasp larvae feed on some, but not all, of the seeds produced by fertilization. Most of the 700 fig plants known are each pollinated by only one or a few specific wasp species (3). These complex relationships are one reason pollinator diversity is critical.

Measuring the Success of Conservation Legislation

A bee pollinates flowers in a field. Pollinator diversity is a critical aspect of ecosystems.
A bee pollinates the lavender flowers.

We are now beginning to recognize how critical pollinator diversity is to our own survival, and many governments, from the local level to the national level are enacting policies and legislation to help protect endangered or threatened pollinator species. However, ecosystems and biodiversity are complex subjects that make measuring and attributing meaningful progress on conservation difficult. Not only are there multiple variables in every instance, but determining the baseline starting point before the legislation is difficult. However, there are dramatic examples of success in saving species through legislative and regulatory action. The recovery of the bald eagle and other raptor populations in the United States after banning the use of DDT is one such example (4).

Continue reading “The Buzz on Biodiversity: Exploring Pollinator Diversity Through Mitochondrial DNA Analysis”

Ancient Retroviruses and Modern Cancer: Role of Endogenous Retroviruses in Transcriptional Changes in Tumor Cells 

Approximately 30 million years ago, a retrovirus integrated into the germline of a common ancestor of baboons, gorillas, chimpanzees and humans. That endogenous retrovirus, now known as gammaretrovirus human endogenous retrovirus 1 (HERV-1), may provide clues about the aberrant regulation of gene transcription that enables tumor cells to grow and survive.  

Understanding the Mechanism Behind Cancer Gene Expression 

Scientists have long described the striking differences in gene expression, signaling activity and metabolism between cancer cells and normal cells, but the underlying mechanisms that cause these differences are not fully understood. In a recent Science Advances article, published by Ivancevic et al., researchers from the University of Colorado, Boulder; the University of Colorado Anschutz Medical Campus, and the University of Colorado School of Medicine report their efforts to identify endogenous retrovirus elements that might be part of the answer to the complex question of what biological events are responsible for the changes in gene expression in cancer cells.  

The researchers hypothesized that transposable elements (TEs), specifically those associated with endogenous retroviruses could be involved in cancer-specific gene regulation.  Endogenous retroviruses (ERVs) are the remnants of ancient retroviral infections that have integrated into the germline of the host. 

The transposable element LTR10, derived from an endogenous retrovirus, can alter gene expression in a number of cancers. Artist's conception of an invasive cancer cell.

Identifying Endogenous Retrovirus Elements That Affect Cancer Gene Expression 

Continue reading “Ancient Retroviruses and Modern Cancer: Role of Endogenous Retroviruses in Transcriptional Changes in Tumor Cells “