Studying Mitochondrial Fission with NanoBiT Complementation Assay

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Vote for NanoBiT™ in the Scientist’s Choice Awards and get a chance to win a $500.00 US Amazon voucher from Select Science.

Motivation
It’s a new year. Whether you’re a self-improvement fanatic or just ready for good things to start happening, you’ve got a plan. You might be changing up an old exercise routine or trying a new cooking technique.

And at work, you are digging deeper; this is the year you illuminate the protein interactions that you’ve previously not been able to visualize.

Good news. There is a new protein complementation assay that can help. Studying-mitochondrial-fission-poster

About NanoBiT
NanoBiT™ Complementation Reporter is a recently developed protein interaction assay that features the improved NanoLuc® luciferase. NanoLuc, originally isolated from a deep sea shrimp, is a small luciferase that provides a much brighter signal than firefly luciferase.

About Split Luciferase Systems
If you’re interrogating two proteins to understand the conditions under which they interact, a split luciferase system enables you to tag each protein with a luciferase subunit. Interaction of the tagged proteins facilitates the complementation of the subunits, resulting in a luminescent signal. Continue reading

Key Advances in PPI Research

small linkedin thumbnailOur understanding of the microscopic world has been shaped by the tools available to monitor and visualize cellular interactions. We “stand on the shoulders of giants” to propel our research to even greater heights. Studying protein-protein interactions (PPI) has proved fruitful for our understanding of cellular metabolism, signal transduction, and more. Scientists are starting to build whole organism interactomes (kindred to the metabolome and genome) that could have huge implications towards understanding and treating disease. Let us take a trip down memory lane to see where we have come from.  Continue reading