Harnessing qPCR and RT-qPCR in Your Laboratory

qPCRQuantitative PCR (qPCR) and reverse transcription qPCR (RT-qPCR) are useful tools in laboratories around the world for absolute or relative quantification of DNA or RNA targets from biological samples. Once you understand the basic principles and have optimized the reaction and cycling parameters, these techniques tend to be rapid, accurate and easy-to-perform—makes me wish that qPCR and RT-qPCR were more commonplace when I worked in the lab and used radioactive Northern blots to quantitate my gene of interest. This is clearly another case of “If only I had known then what I know now”. While I’m no longer working in the lab, it’s not too late for you to benefit from a good introduction to qPCR and RT-qPCR, and on January 15, 2013, that’s exactly what one Promega Research and Development Scientist presented: A webinar entitled “Optimize Your qPCR and RT-qPCR Assays with Careful Planning and Design”.
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The Keys to Successful Transfection

Transfection, like many techniques in the lab, can be an art, especially for stubborn cell lines. To maximize transfection efficiency, you must have the right conditions and, for some cell lines it seems, the right touch. If you’re lucky, you are working with less finicky cells that transfect well regardless of conditions. If you’re not so lucky, you might want to spend a few minutes reading through this list of ways that you can improve your transfection results.
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