Methods for Determining DNA Yield and Concentration

Determining DNA Yield and Purity

This post is provided as a general introduction to common laboratory methods for determining the yield and purity of purified DNA samples. DNA yield can be assessed using various methods including absorbance (optical density), agarose gel electrophoresis, or use of fluorescent DNA-binding dyes.  All three methods are convenient, but have varying requirements in terms of equipment needed, ease of use, and calculations to consider.

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