The latest Promega Webinar covered trypsin and protein analysis for mass spectrometry. Here we summarize some of the discussion from this webinar.
Guest Blog by Lynn Litterer, Promega Technical Services Scientist
You are studying your favorite biological system and fascinating questions about how it works. Chances are good that some of those questions involve protein interaction with other proteins and molecules in the cell. Where and when proteins are active is often regulated by post-translational modification. Mass spectrometry (mass spec, MS) is a powerful tool to identify protein sequence and modifications. Strategic use of mass spec tools can substantially improve the analysis of your samples.
Why is trypsin the first choice for protein sequencing? Continue reading