Proteomics, the analysis of the entire protein content of a living system, has become a vital part of life science research, and mass spectrometry (MS) is the method for analyzing proteins. MS analysis of protein content allows researchers to identify proteins, sequence them and determine the nature of post translational modifications. Mass spectrometry allows characterization […]
The complexity of biological samples places high demand on mass spec analytical capability. Adequate monitoring of instrument performance for proteomics studies requires equally complex reference material such as whole-cell extracts. However, whole-cell extracts available commercially are developed for general research (e.g., enzymatic or Western blot analysis) and contain detergents and salts that interfere with reverse […]
One of the approaches to identify proteins by mass spectrometry includes the separation of proteins by gel electrophoresis or liquid chromatography. Subsequently the proteins are cleaved with sequence-specific endoproteases. Following digestion the generated peptides are investigated by determination of molecular masses or specific sequence. For protein identification the experimentally obtained masses/sequences are compared with theoretical […]
Sometimes, when using trypsin to study a protein sequence or protein modifications, sequence coverage just isn’t quite as complete as you’d like. Looking for a protease with novel cleavage specificity or a protease that functions well in a low pH environment? Promega has a protease for that. ProAlanase is a new site-specific endoprotease that preferentially […]
The largest contiguous population of elephants in Africa lives in the Kavango-Zambezi Trans Frontier Conservation Area (KAZA TFCA) which encompasses parts of Botswana Zimbabwe, Zambia, Angola and Namibia. Within KAZA, nearly 90% of the elephant population is concentrated in Botswana (58%) and Zimbabwe (29%). In June of 2020, over 300 elephants were found dead in […]
The new Spectrum CE System supports future 8-color technology while maintaining compatibility with existing 5- and 6-color kits—even ones that Promega does not sell.
Today’s guest blog about the 2020 virtual iGEM Giant Jamboree is written by Abigail Conner, Co-Team Leader of iGEM King’s College London (KCL). In October 2019, I returned to London from Boston feeling elated after an unforgettable week at the Giant Jamboree. My team, Capacity, had just won a Silver Medal. I had the privilege […]
In older people, low muscle mass is strongly associated with reduced functional capacity and an increased risk of disability. Myostatin is a negative regulator of muscle growth and has become an important target for pharmaceutical companies designing therapeutics to address age-associated muscle loss. Anti-myostatin drugs increase muscle size and strength in preclinical studies. Fortetropin is […]
The rapid advancement of next-generation sequencing technology, also known as massively parallel sequencing (MPS), has revolutionized many areas of applied research. One such area, the analysis of mitochondrial DNA (mtDNA) in forensic applications, has traditionally used another method—Sanger sequencing followed by capillary electrophoresis (CE). Although MPS can provide a wealth of information, its initial adoption […]
Dennis Dimick has focused his journalism career on the collision between human aspiration and the planet. The son of fisheries biologists, Dimick grew up on a farm in Oregon’s Willamette Valley, and he holds degrees in agriculture and agricultural journalism from Oregon State University and the University of Wisconsin-Madison. In his 35 years at National […]
