It’s All in the Details: Simplified and Improved miRNA Purification from Tissue

miR-133 microRNA (green) and myogenin mRNA (red) in differentiating C2C12 cells. Image by Ryan Jeffs, courtesy of Wikimedia Commons.

miR-133 microRNA (green) and myogenin mRNA (red) in differentiating C2C12 cells. Image by Ryan Jeffs, courtesy of Wikimedia Commons.

Consider the microRNA. At only about 21–26 nucleotides in length, microRNAs (miRNAs) are short, but don’t dismiss miRNAs as too short to accomplish much of anything. miRNAs are a multifunctional workhorse that play a key role in a number of genetic regulatory mechanisms throughout the plant and animal kingdoms and even in certain viruses. Scientists estimate that the human genome encodes about 2,000 different miRNAs and miRNAs account for about 3–4% of human genes (1).

Next Tuesday, we invite you to consider the microRNA with us as we host a webinar discussing the growing field of miRNA research and highlight a new, simplified miRNA purification method. Follow the link below to register.

It’s All in the Details (or Small RNA): Simplified and Improved miRNA Purification from Tissue

Presented by Douglas Horejsh, Ph.D.
Tuesday, January 27

Reference

  1. Valinezhad Orang, A., Safaralizadeh, R. and Kazemzadeh-Bavili, M. (2014) Mechanisms of miRNA-mediated gene regulation from common downregulation to mRNA-specific upregulation. Int. J. Genomics Article ID 970607 http://dx.doi.org/10.1155/2014/970607
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Kelly Grooms

Scientific Communications Specialist at Promega Corporation
Kelly earned her B.S. in Genetics from Iowa State University in Ames, IA. Prior to coming to Promega, she worked for biotech companies in San Diego and Madison. Kelly lives just outside Madison with her husband, son and daughter. Kelly collects hobbies including jewelry artistry, reading, writing, photography and knitting. She would like to be an avid runner, as evidenced by her growing collection of running gear and her single half-marathon finishers t-shirt.

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