In today’s biotech landscape, speed and precision are essential. For Tridek-One Therapeutics, a Paris-based spin-off from INSERM founded in 2018, these qualities drive their mission to develop first-in-class CD31 checkpoint agonist therapies for autoimmune and inflammatory diseases. By leveraging CD31’s ITIM motifs to modulate ITAM signaling, their approach targets immune cells selectively, reducing the risk of broad immunosuppression.
Operating in a biotech incubator with limited space and shared equipment, the team—including Trang Tran, PhD, Preclinical Research Director, and Guillaume Even, Senior Laboratory Technician—depends on luminescent assays requiring both sensitivity and precise timing. Relying on a shared plate reader often delayed extracellular ATP assays that needed rapid measurement. Walking between lab spaces and potentially waiting for access to the plate reader was not feasible.
Tridek-One needed a dedicated, reliable luminometer that could support their time-sensitive workflow and fit into their small lab space. That’s when Tridek-One discovered the MyGlo® Reagent Reader, Promega’s compact, portable 96-well luminometer and transformed their workflow. Even noted that, when they first tried MyGlo®, they “directly saw the power of this small machine.” Tran and Even found that MyGlo®’s performance and sensitivity were comparable to more expensive multi-mode readers, which gave them confidence in choosing MyGlo® as a reliable and cost-effective solution. Because they prefer to use 96-well microplates, MyGlo® fit their experimental setup perfectly.
Compact Design, Big Impact
MyGlo® provided independence, reliability, and speed—with a footprint slightly larger than a standard microtiter plate. “The fact that it’s on our bench, that we can use it very quickly…and the fact that it’s portable and can be easily transported to other locations is great,” Tran said. “For us, there are only positive things in this small machine,” added Even.
MyGlo®’s portability expanded possibilities. The Tridek-One team brought their MyGlo® to partner labs that didn’t have luminescence plate readers, enabling experiments that otherwise would have been delayed or infeasible. Each time, the device reconnected smoothly through Promega’s cloud-based ProNect® Data Platform.
Data Anywhere, Anytime
But the impact isn’t limited to portability—through ProNect®, MyGlo® also transforms how data is accessed. MyGlo®’s integration with the cloud-based ProNect® Data Platform has been invaluable for Tridek-One. With ProNect®, the team accesses data from any location—Paris, home, or collaborator labs. The ability to access their data in the cloud (ProNect®) from any computer and any location is a key feature for Tran and Even. This capability not only streamlines collaboration but ensures that their data remains organized and accessible wherever their experiments take them. For a team balancing multiple projects and collaborators, this flexibility keeps projects moving without time spent searching for files.
Real-Time Confidence in Results
MyGlo® is now an important component of Tridek-One’s workflow. They read luminescence from the RealTime-Glo® Extracellular ATP Assay and several Lumit® Cytokine Immunoassays to evaluate the impact of their bispecific antibodies targeting ITAM receptors and CD31. ATP release confirms whether compounds effectively block the intended ITAM pathway, while Lumit® assays measure expression of cytokine targets after treatment.
The workflow is streamlined: plates are read, and data is displayed instantly as a heatmap, enabling them to evaluate results quickly before proceeding with additional experiments. Even described the benefits of the user interface, “It’s a clear view of the data…you see in a second whether you did it well or not.” Features such as the ability to quickly download results after a read cut down on extra steps, saving valuable time in a day packed with experiments and data analysis.
User-Friendly by Design
MyGlo®’s ease of use and ProNect® Data Platform’s intuitive interface and streamlined workflow save time when onboarding new Tridek-One team members. The team noted that it’s user friendly, so training takes less than 10 minutes.
In an incubator environment where every minute counts and flexibility is essential, Tridek-One found more than just a compact luminometer in MyGlo® Reagent Reader. They gained independence, ensured data quality, and extended their science beyond the limits of their lab. Even offered high praise for MyGlo®, “We love it. You cannot have built a better thing.”
For scientists searching for reliable, portable luminescence detection, Tridek-One’s experience shows how the right tool can alleviate bottlenecks and accelerate discovery. What impact could MyGlo® and the ProNect® Data Platform have on your workflow? Learn more here.
RealTime-Glo, Lumit, ProNect, and MyGlo are registered trademarks of Promega Corporation.
For research use only. Not for use in diagnostic procedures.
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