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Workflow for the first steps of Combined Bisulfite Restriction Analysis, starting with bisulfite conversion which selective converts unmethylated cytosine to uracil, followed by methylation-independent PCR, and finally restriction digestion of the PCR products. Digested fragments are then quantified, and DNA methylation levels can be determined for the input sample. Image from Wikimedia Commons: Attribution under Creative Commons License: http://en.wikipedia.org/wiki/File:Cobra_workflow.svg

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