Efficient Cloning and Expression of High Protein Yields Using KRX Cells

Escherichia coli remains the first choice of many researchers for producing recombinant protein for functional studies due to its ease of use, well established protocols, rapid cell growth and low cost of culturing. Researchers often need to clone using an E. coli host with good transformation characteristics first, then transfer the desired clone to the expression host. We have developed a new E. coli host KRX, that provides protein yields comparable to those of BL21(DE3) but with much higher transformation efficiencies. Continue reading “Efficient Cloning and Expression of High Protein Yields Using KRX Cells”