You’ve used Promega products, and talked to Promega Technical Services Scientists or found helpful protocols and support information at www.promega.com
Did you know that there is also a webinar series to assist you in the use of Promega products?
www.promega.com/webinars provides a schedule of upcoming webinars. In addition there are links to previous webinars that allow you to either view the recording or download a pdf of the presentation. There is also a pdf of additional material available for each past webinar.
To register for a webinar, use the “registration” link at www.promega.com/webinars This allows you to not only view the webinar, but also to participate in the live chat. Need a reminder? You can also sign-up for monthly invitations to webinars at the webinars page (see link above).
The live chat is monitored by Promega Technical Services Scientists. Type your question into the chat window and a Technical Services Scientist will provide an answer. In fact, the webinar presenter sometimes also logs in and participates in the live chat.
Of course if you are viewing a webinar after the scheduled presentation, there is no live chat. But you will be able to see the chat that accompanied that presentation and see the interesting question and answer exchanges between customers and Technical Services Scientists.
Promega webinars are designed to provide tips and techniques to support your research. Webinars are also a great way to learn about new Promega products.
For instance, a recent webinar was: “NanoLuc™: A Smaller, Brighter and More Versatile Luciferase Reporter”. This webinar, presented by Promega Research and Development Scientist Dr. Brock Binkowski, explains the features of an exciting new luciferase, as well as information on how the luciferase was developed and how it differs from Firefly and Renilla luciferases.
Here is a sample of the chat from this June 12, 2012 NanoLuc™ Luciferase webinar:
Q: I was just wondering how you control for transfection efficiency, there has been no mention of something like renilla which is used in firefly luc systems to normalise the transfection efficiency of the cells?
A: Hi. We don’t have a dual reporter assay for this (NanoLuc™) luciferase yet. So at this time, you would need to use a separate assay (i.e. separate samples) to do a different (control) reporter. You may also consider using a viability assay to monitor cell counts.
Q: And could the NanoGlo be suitable for medium containing phenol red?
A: No problem with use of medium that contains phenol red.
Q: In the data of Nluc-B2AR, so the Nluc also could apply to live cell imaging assay?
A: Yes, Nluc is very well suited for live cell imaging applications where furimazine (the substrate for NanoLuc™ luciferase) is added directly to the cells. An image of Nluc-B2AR fusion was shown (in data with this webinar).
Q: Which kind of reporter lysis buffer could be used with Nluc? Because the cell will be seeding in a 6-well plate.
A: Use Nano-Glo™ Luciferase Assay Reagent with NanoLuc™ Luciferase. No problem to use in 6-well format. Nano-Glo™ Reagent contains everything needed to lyse the cells.
The chat during the Promega webinars is lively, with helpful questions and answers. Having said that, a note that each webinar presenter will encourage you to listen carefully during the presentation and ask questions at the end, so as to not miss any of the information.
Remember that Promega has Technical Services Scientists worldwide, so no matter where you live, Technical Support is available to answer your questions, during the webinars or any time a question arises.
The next Promega webinar is Tuesday, July 10, 2012. The title is: “”Beyond Trypsin: Strategies to Improve Mass Spec Sequence Coverage and PTM Analysis”, by Mike Rosenblatt, Ph.D.
Go to www.promega.com/webinars to sign up.
See you there.
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