In-gel Protein Digestion: Increase Protein Identifications with an Abridged Procedure

10827MAIn-gel digestion complete in only 1 hour.[/caption]Identification of proteins resolved by SDS-PAGE requires a lengthy in-gel digestion and extraction procedure. The publication, Mass Spectrometry Compatible Surfactant for the Optimized In-Gel Protein Digestion, Saveliev, S. et al. (2013) Anal. Chem. 85, 907–14, addressed these obstacles by illustrating the technical benefits of the ProteaseMAX™ mass spectrometry surfactant.  A recent webinar reviewed key aspects of that paper that included:

11253MDImproved peptide recovery from gels: ProteaseMAX™ improved identification of proteins by enhanced protein digestion, increased peptide extraction and minimized postdigestion peptide loss. This is a major benefit for the in-gel digestion of low abundant proteins and enables the use of minimal sample material.

The webinar also contained information regarding the how the structure of ProteaseMAX™ Surfactant enables it to compatible with mass spec and how it can be used for protein denaturation and solubilization.

About the Webinar Series
www.promega.com/webinars/ provides a schedule of upcoming webinars. In addition, there are links to previous webinars, which allow you to view the recording or download a pdf of the presentation. There is also a pdf of additional material available for each past webinar.

To register for a webinar, use the Registration link at: www.promega.com/webinars/ This allows you to view a live webinar and participate in the live chat.

Need a reminder? You can also sign-up for monthly invitations to webinars at the webinars page (see link above). Note: Live chat is only available for live webinars, not recorded webinars.

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Gary Kobs

Strategic Marketing Manager at Promega Corporation
Gary earned his B.S. in Bacteriology, UW-Madison in 1982. From 1982–1986 he served as Research Tech at UW-Madison. From 1986 to the present Gary has been with Promega Corporation serving in many capacities including as the very first editor of Promega Notes. He was also Manager Tech Services and Training, Product Manager Restriction/Modifying Enzymes, Product Manager Protein Analysis, and is now Marketing Manager Protein Analysis.

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