How to use Cell Viability and Cytotoxicity Assays Together to Get Real-Time Answers about Cell Death

Real-time measurement of cytotoxicity

CellTox™ Green Dye Binds DNA Released By Dying Cells

If you have ever wondered about the differences between the various cell viability and cytotoxicity assays available, why you would choose one over another or how they can be used together, tune in to the webinar “A Real-Time Cytotoxicity Assay That Delivers More Relevant Data” (Tuesday, Sept 10). In this webinar, Promega Scientist Drew Niles explains how various metabolism and biomarker-based viability and cytotoxicity assays work, and describes how they can be used most effectively to give maximum information about mechanism and timing of cell death.

“Is a cell treatment toxic?” and “Why are the cells dying?” are questions that can be difficult to answer simply. The answer depends on dosage, treatment time, mechanism of action of the test compound, and the cell type used—and may sometimes be limited by features of the assay itself. For example, many viability and cytotoxicity assays measure biomarkers that are themselves subject to degradation over the course of longer experiments, complicating the interpretation of results. Drew provides an explanation of these issues and illustrates the critical role of timing in deciding on the assay to use and in interpreting results.

Drew will also cover how the CellTox™ Green assay—a new cytotoxicity assay that measures DNA release from dead/dying cells—can be used to continuously monitor changes in cell viability over time. The assay is non-toxic, allowing multiple viability timepoints from a single sample, giving a picture of cell health in great detail over the course of an experiment. Because the CellTox™ Green Assay reagent is non-toxic it also allows easy multiplexing of the cytotoxicity assay with other cell health assays.

Why multiplex? Drew will answer this question and provide example assay data showing exactly how real-time measurement of cytotoxicity, combined with measurement of cell viability markers and apoptosis assays can be used very effectively to gain valuable information about the mechanism of action of different test compounds in a single experiment.

The Webinar will last about 45 minutes and will include live chat to allow your questions to be addressed directly. Check it out if you are looking for answers on how to best use the various assay methods available to gain information about how, when and why cells die after exposure to test compounds, biologicals, transgenes or other potentially toxic events.

About the Webinar Series

A schedule of upcoming webinars can be found at: www.promega.com/webinars/. In addition, there are links to previous webinars, which allow you to view the recording or download a pdf of the presentation. There is also a pdf of additional material available for each past webinar.

To register for a webinar, use the Registration link at: www.promega.com/webinars/ This allows you to view a live webinar and participate in the live chat.

Need a reminder? You can also sign-up for monthly invitations to webinars at the webinars page (see link above). Note: Live chat is only available for live webinars, not recorded webinars.

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Isobel Maciver

Isobel is a graduate of the University of Edinburgh and of Aston University in Birmingham, U.K. She is a technical writer and editor, and is also manager of the Scientific Communications group at Promega. She enjoys writing about issues in science and communication.

2 thoughts on “How to use Cell Viability and Cytotoxicity Assays Together to Get Real-Time Answers about Cell Death

  1. Hi,

    I would very much like to watch this webinar, but the link does not seem to work. Could you please provide a new link to the webinar or an alternative location where it can be viewed?

    Best regards,

    Jarle Bruun

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